Rabbit ATF5 Polyclonal Antibody | anti-ATF5 antibody

ATF5 Rabbit pAb

Cat. Num. AAA4751811

• Gene Names: ATF5; ATFX; HMFN0395
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: ATF5; Polyclonal Antibody; ATF5 Rabbit pAb; Cyclic AMP-dependent transcription factor ATF-5; ATFX; HMFN0395; anti-ATF5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Widely expressed with higher expression levels in liver.
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-ATF5 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; IHC: 1:20-1:500; ICC/IF: 1:50; FC/FACS: 1:20; IP: 1:20
• Immunogen: A synthetic peptide of human ATF5
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cytoplasm, Cytoskeleton, Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of ATF5 in K562,Rat Brain,C6,Hela cell lysates using ATF5 Rabbit pAb(1:1000 diluted). Predicted band size:31kDa. Observed band size:31kDa.)

Related Product Information for anti-ATF5 antibody

Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631). Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4 (PubMed:15358120). Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate. Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation. Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes. May be involved in osteogenic differentiation (PubMed:22442021). Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1 (PubMed:21791614). Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent. Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver (PubMed:18332083). In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase (PubMed:22528486, PubMed:18701499). May act as a negative regulator of IL1B transduction pathway in liver (PubMed:24379400). Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members (PubMed:25512613). Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation (PubMed:24216764). Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material (PubMed:26213385).

NCBI and Uniprot Product Information

• NCBI GI #: 302370956
• NCBI GeneID: 22809
• NCBI Accession #: NP_001180575.1
• NCBI GenBank Nucleotide #: NM_001193646.1
• UniProt Accession #: Q9Y2D1; Q9BSA1; Q9UNQ3; B3KND3
• Molecular Weight: 30,674 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-5
• NCBI Official Synonym Full Names: activating transcription factor 5
• NCBI Official Symbol: ATF5
• NCBI Official Synonym Symbols: ATFX; HMFN0395
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-5; transcription factor ATFx; cAMP-dependent transcription factor ATF-5
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-5
• Protein Family: Cyclic AMP-dependent transcription factor
• UniProt Gene Name: ATF5
• UniProt Synonym Gene Names: ATFX; cAMP-dependent transcription factor ATF-5
• UniProt Entry Name: ATF5_HUMAN

Uniprot Description

ATF-5: Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Belongs to the bZIP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm

Molecular Function: protein binding; heat shock protein binding; transcription corepressor activity

Biological Process: circadian rhythm; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; olfactory bulb interneuron development; multicellular organism growth; negative regulation of astrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of apoptosis; post-embryonic development