Canine Histamine ELISA Kit | HIS elisa kit

Canine Histamine ELISA Kit

Cat. Num. AAA28306

• Gene Names: HRH2; H2R
• Reactivity: Canine
• Synonyms: Histamine; Canine Histamine ELISA Kit; HIS elisa kit

• Reactivity: Canine
• Sequence Length: 397

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Detection Range: 1.25 ng/ml ~ 40 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Canine HIS in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Canine HIS concentration. The concentration of Canine HIS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 197085610
• NCBI GeneID: 3274
• NCBI Accession #: NP_001124527.1
• NCBI GenBank Nucleotide #: NM_001131055.1
• UniProt Accession #: P25021; Q14464; Q7Z5R9; B5BUP7
• Molecular Weight: 40,098 Da
• NCBI Official Full Name: histamine H2 receptor isoform 1
• NCBI Official Synonym Full Names: histamine receptor H2
• NCBI Official Symbol: HRH2
• NCBI Official Synonym Symbols: H2R
• NCBI Protein Information: histamine H2 receptor; HH2R; gastric receptor 1; gastric receptor I
• UniProt Protein Name: Histamine H2 receptor
• Protein Family: Histamine oxidase
• UniProt Gene Name: HRH2
• UniProt Synonym Gene Names: H2R; HH2R
• UniProt Entry Name: HRH2_HUMAN

NCBI Description

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

H2R: The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: histamine receptor activity

Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; gland development; gastrin-induced gastric acid secretion; gut development; histamine-induced gastric acid secretion; positive regulation of vasoconstriction; visual learning; regulation of synaptic plasticity; immune response; memory