Goat MLX / TCFL4 Polyclonal Antibody | anti-MLX antibody

Goat Polyclonal to Human MLX / TCFL4

Cat. Num. AAA244142

• Gene Names: MLX; MAD7; MXD7; TCFL4; bHLHd13
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rat; Predicted Reactivity: Goat (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MLX / TCFL4; Polyclonal Antibody; Goat Polyclonal to Human MLX / TCFL4; Anti-MLX / TCFL4 Antibody (Internal) IHC-plus; BHLHd13; BigMax protein; MXD7; MAD7; Max-like bHLHZip protein; MLX; MAX dimerization protein; Protein BigMax; Max-like protein X; Transcription factor-like 4; Human MLX; TCFL4; anti-MLX antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rat; Predicted Reactivity: Goat (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human MLX. This antibody is expected to recognize all three reported isoforms (NP_937848.1; NP_937847.1; NP_733752.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 298

• Applicable Applications for anti-MLX antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA, IHC-P (2.5 - 3.75 ug/ml); Usage: Western Blot: Preliminary experiments in Human, Mouse and Rat Liver lysates gave no specific signal but low background (at antibody concentration up to 1 ug/ml).
• Target Species: Human
• Immunogen Description: Synthetic peptide C-QIVKAHQDNPHEGED from an internal region of human MLX (NP_937848.1; NP_937847.1; NP_733752.1).
• Immunogen Type: Synthetic peptide
• Immunogen: MLX / TCFL4 antibody was raised against synthetic peptide C-QIVKAHQDNPHEGED from an internal region of human MLX (NP_937848.1; NP_937847.1; NP_733752.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum (100%); Goat (93%); Rabbit, Turkey, Chicken, Platypus (87%); Lizard, Xenopus (80%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MLX antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 24586667
• NCBI GeneID: 6945
• NCBI Accession #: NP_733752.1
• NCBI GenBank Nucleotide #: NM_170607.2
• UniProt Accession #: Q9UH92; Q53XM6; Q96FL2; Q9H2V0; Q9H2V1; Q9H2V2; Q9NXN3; A8K2J3; B2RAV8; B2RD73
• Molecular Weight: 27,883 Da
• NCBI Official Full Name: max-like protein X isoform gamma
• NCBI Official Synonym Full Names: MLX, MAX dimerization protein
• NCBI Official Symbol: MLX
• NCBI Official Synonym Symbols: MAD7; MXD7; TCFL4; bHLHd13
• NCBI Protein Information: max-like protein X; BigMax protein; MAX-like bHLHZIP protein; transcription factor-like protein 4; class D basic helix-loop-helix protein 13
• UniProt Protein Name: Max-like protein X
• Protein Family: Max-like protein
• UniProt Gene Name: MLX
• UniProt Synonym Gene Names: BHLHD13; TCFL4; bHLHd13
• UniProt Entry Name: MLX_HUMAN

NCBI Description

The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This gene product may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MLX: Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; energy reserve metabolic process; positive regulation of cellular metabolic process; nucleocytoplasmic transport; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent