Rabbit ATF2 Polyclonal Antibody | anti-ATF2 antibody

Anti-ATF2 Antibody

Cat. Num. AAA415643

• Gene Names: GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot
• Purity: Protein A purified
• Synonyms: ATF2; Polyclonal Antibody; Anti-ATF2 Antibody; anti-ATF2 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATF2 Antibody can detect endogenous ATF2 protein. Not tested with other proteins
• Purity/Purification: Protein A purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 211

• Applicable Applications for anti-ATF2 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended dilutions:; Peptide ELISA: 1:80,000; WB: 1:500 - 1:2000; Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-ATF2 antibody

This gene encodes a highly conserved neurotrophic factor.The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection)protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.

References

Hoeffler,J.P., Lustbader,J.W. and Chen,C.Y Identification of multiple nuclear factors that interact with cyclic adenosine 3',5'-monophosphate response element-binding protein and activating transcription factor-2 by protein-proteininteractions

NCBI and Uniprot Product Information

• NCBI GI #: 4503975
• NCBI GeneID: 2668
• NCBI Accession #: NP_000505
• NCBI GenBank Nucleotide #: NM_000514.3
• Molecular Weight: 18,123 Da
• NCBI Official Full Name: glial cell line-derived neurotrophic factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: glial cell derived neurotrophic factor
• NCBI Official Symbol: GDNF
• NCBI Official Synonym Symbols: ATF1; ATF2; HSCR3; HFB1-GDNF
• NCBI Protein Information: glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor
• UniProt Protein Name: Glial cell line-derived neurotrophic factor
• Protein Family: Alcohol O-acetyltransferase
• UniProt Gene Name: GDNF
• UniProt Entry Name: GDNF_HUMAN

NCBI Description

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]

Uniprot Description

GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5p13.1-p12

Cellular Component: extracellular region

Molecular Function: protein homodimerization activity; growth factor activity; receptor binding

Biological Process: nervous system development; positive regulation of dopamine secretion; axon guidance; peristalsis; adult locomotory behavior; mRNA stabilization; positive regulation of monooxygenase activity; regulation of dopamine uptake; signal transduction; enteric nervous system development; sympathetic nervous system development; regulation of gene expression; ureteric bud branching; induction of an organ; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; postganglionic parasympathetic nervous system development; negative regulation of neuron apoptosis; positive regulation of cell differentiation; postsynaptic membrane organization; neural crest cell migration; metanephros development; neurite development; negative regulation of apoptosis

Disease: Central Hypoventilation Syndrome, Congenital; Hirschsprung Disease, Susceptibility To, 3; Pheochromocytoma