Rabbit anti-Human ATF1 Polyclonal Antibody | anti-ATF1 antibody

ATF1, CT (ATF1, Cyclic AMP-dependent transcription factor ATF-1, Activating transcription factor 1, Protein TREB36) (PE)

Cat. Num. AAA6275830

• Gene Names: ATF1; TREB36; EWS-ATF1; FUS/ATF-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATF1; Polyclonal Antibody; CT (ATF1; Cyclic AMP-dependent transcription factor ATF-1; Activating transcription factor 1; Protein TREB36) (PE); Protein TREB36; anti-ATF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ATF1 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ATF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ATF1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATF1 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). ATF1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(ATF1 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ATF1 antibody

Antibodies; Transcription Factors

References

Wang, W.L., et al. Mod. Pathol. 22(9):1201-1209(2009). Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009). Zheng, D., et al. Cancer Res. 68(18):7650-7660(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4885073
• NCBI GeneID: 466
• NCBI Accession #: NP_005162.1
• NCBI GenBank Nucleotide #: NM_005171.4
• UniProt Accession #: P18846; P25168; Q9H4A8; AAB25878
• Molecular Weight: 29,233 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-1
• NCBI Official Synonym Full Names: activating transcription factor 1
• NCBI Official Symbol: ATF1
• NCBI Official Synonym Symbols: TREB36; EWS-ATF1; FUS/ATF-1
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-1; cAMP-dependent transcription factor ATF-1
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-1
• Protein Family: Alcohol O-acetyltransferase
• UniProt Gene Name: ATF1
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-1
• UniProt Entry Name: ATF1_HUMAN

NCBI Description

This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]

Uniprot Description

ATF-1: a transcription factor that is a member of the leucine zipper family. Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Binds the Tax-responsive element (TRE) of HTLV-I. Activated downstream of IL-1. c-Src and TRAF6 are mediators of IL-1-induced AP-1 activation. Plays an important role in the trans-activation of the MHC class II trans-activator (CIITA) promoter III in B cells.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein heterodimerization activity; protein complex binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; cellular protein complex assembly; response to cobalt ion; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; positive regulation of DNA replication