Human CENPF ELISA Kit | CENPF elisa kit
Human CENPF (Centromere Protein F) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CENPF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CENPF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CENPF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CENPF. You can calculate the concentration of Human CENPF in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]
Uniprot Description
CENPF: a nuclear matrix protein that assembles onto kinetochores at late G2. Is hyperphosphorylated during mitosis and rapidly degraded after mitosis. Involved in chromosome segregation during mitosis. Interacts with retinoblastoma protein (RB), CENP-E and BUBR1. Two alternatively spliced human isoforms have been described.
Protein type: Transcription, coactivator/corepressor; Nuclear envelope; Cell cycle regulation
Chromosomal Location of Human Ortholog: 1q41
Cellular Component: spindle pole; nuclear matrix; pronucleus; nuclear envelope; cytosol; nucleoplasm; kinetochore; perinuclear region of cytoplasm; cytoplasm; spindle; outer kinetochore of condensed chromosome; midbody; chromatin; nucleus; chromosome, pericentric region
Molecular Function: protein C-terminus binding; dynein binding; protein binding; protein homodimerization activity; chromatin binding; transcription factor binding
Biological Process: response to drug; mitosis; muscle development; kinetochore assembly; regulation of cell cycle; chromosome segregation; cell proliferation; protein transport; cell division; regulation of G2/M transition of mitotic cell cycle; mitotic cell cycle spindle assembly checkpoint; metaphase plate congression; mitotic cell cycle; cell differentiation; negative regulation of transcription, DNA-dependent; G2/M transition of mitotic cell cycle; regulation of striated muscle development
Disease: Ciliary Dyskinesia, Primary, 31
