Human ATPase family AAA domain containing protein 2 (ATAD2) ELISA Kit | ATAD2 elisa kit

Human ATPase family AAA domain containing protein 2 (ATAD2) ELISA Kit

Cat. Num. AAA7204076

• Gene Names: ATAD2; ANCCA; CT137; PRO2000
• Reactivity: Human
• Synonyms: ATPase family AAA domain containing protein 2 (ATAD2); Human ATPase family AAA domain containing protein 2 (ATAD2) ELISA Kit; ATAD2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATAD2. No significant cross-reactivity or interference between ATAD2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATAD2 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATAD2 elisa kit

Intended Uses: This ATAD2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATAD2. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: ATAD2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATAD2 antibody and an ATAD2-HRP conjugate. The assay sample and buffer are incubated together with ATAD2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATAD2 concentration since ATAD2 from samples and ATAD2-HRP conjugate compete for the anti-ATAD2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATAD2 from the sample, fewer sites are left to bind ATAD2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATAD2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 24497618
• NCBI GeneID: 29028
• NCBI Accession #: NP_054828.2
• NCBI GenBank Nucleotide #: NM_014109.3
• Molecular Weight: 47,986 Da
• NCBI Official Full Name: ATPase family AAA domain-containing protein 2
• NCBI Official Synonym Full Names: ATPase family, AAA domain containing 2
• NCBI Official Symbol: ATAD2
• NCBI Official Synonym Symbols: ANCCA; CT137; PRO2000
• NCBI Protein Information: ATPase family AAA domain-containing protein 2; AAA nuclear coregulator cancer-associated protein
• UniProt Protein Name: ATPase family AAA domain-containing protein 2
• Protein Family: ATPase family AAA domain-containing protein
• UniProt Gene Name: ATAD2
• UniProt Synonym Gene Names: L16; PRO2000; ANCCA
• UniProt Entry Name: ATAD2_HUMAN

NCBI Description

A large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq, Jul 2008]

Uniprot Description

ATAD2: a member of the AAA ATPase family. Contains a bromodomain, a domain known to interact specifically with acetylated lysine. Two alternatively spliced isoforms of the human protein have been reported.

Protein type: EC 3.6.1.3; Hydrolase; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: nucleoplasm

Molecular Function: ATPase activity; ATP binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent