Western Blot (WB)
(All lanes: Aspartate aminotransferase, cytoplasmic antibody at 2ug/ml+mouse brain tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 47,45 kDaObserved band size: 46 kDa)
Rabbit anti-Human Aspartate aminotransferase Polyclonal Antibody | anti-GOT1 antibody
Rabbit anti-human Aspartate aminotransferase, cytoplasmic polyclonal Antibody
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Western Blot (WB)
(All lanes: Aspartate aminotransferase, cytoplasmic antibody at 2ug/ml+mouse brain tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 47,45 kDaObserved band size: 46 kDa)
Western Blot (WB)
(All lanes: Aspartate aminotransferase, cytoplasmic antibody at 2ug/ml+mouse brain tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 47,45 kDaObserved band size: 46 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: hepG2 cell,mouse liver,mouse skeletal muscleAll lanes: GOT1 antibody at 4.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47,45 kDaObserved band size: 46 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: hepG2 cell,mouse liver,mouse skeletal muscleAll lanes: GOT1 antibody at 4.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47,45 kDaObserved band size: 46 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using MBS1488479 at dilution of 1:50)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using MBS1488479 at dilution of 1:50)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS1488479 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS1488479 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
NCBI and Uniprot Product Information
NCBI Description
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
Uniprot Description
GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Transferase; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; EC 2.6.1.3; EC 2.6.1.1; Amino Acid Metabolism - alanine, aspartate and glutamate
Chromosomal Location of Human Ortholog: 10q24.1-q25.1
Cellular Component: cytoplasm; cytosol; lysosome; mitochondrion; nerve terminal; nucleus
Molecular Function: aspartate transaminase activity; carboxylic acid binding; cysteine transaminase activity; identical protein binding; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding
Biological Process: 2-oxoglutarate metabolic process; amino acid biosynthetic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; carbohydrate metabolic process; cellular response to insulin stimulus; fatty acid homeostasis; gluconeogenesis; glucose metabolic process; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glutamate metabolic process; glycerol biosynthetic process; methionine salvage; Notch signaling pathway; oxaloacetate metabolic process; polyamine metabolic process; response to glucocorticoid stimulus; sulfur amino acid metabolic process
Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1
Research Articles on GOT1
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Product Notes
The GOT1 got1 (Catalog #AAA1488479) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Aspartate aminotransferase, cytoplasmic polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartate aminotransferase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the GOT1 got1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartate aminotransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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