Rabbit IL2RA Polyclonal Antibody | anti-IL2RA antibody

IL2RA Polyclonal Antibody

Cat. Num. AAA3007127

• Gene Names: IL2RA; p55; CD25; IL2R; IMD41; TCGFR; IDDM10
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IL2RA; Polyclonal Antibody; IL2RA Polyclonal Antibody; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25; anti-IL2RA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IL2RA polyclonal antibody detects endogenous levels of IL2RA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 272

• Applicable Applications for anti-IL2RA antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human IL2RA.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of IL2RA polyclonal antibody at 1:500 dilutionLane1:Thp-1 whole cell lysateLane2:H9C2 whole cell lysateLane3:Raw264. 7 whole cell lysate)

Related Product Information for anti-IL2RA antibody

The IL-2 receptor is amulticomponentcomplexconsisting of three subunits, alpha, beta and gamma, each ofwhich isrequired for high-affinity binding of IL-2. Thealphachain functions primarilyin binding IL-2, whereasthe beta and gamma chainscontribute to IL-2 binding and are essential to IL-2-induced activation ofsignaling pathways leading to T cell growth. Both IL-4R and IL-7R were initially described as single chain, high-affinityligand-binding cytokine receptors. However, it is now well established that the IL-2Rgamma chain functions as a second subunit of the high affinityIL-4R and IL-7R receptors. Consequently, the originally described subunits of these latter receptors are nowreferred to asIL-4Ralphaand IL-7Ralpha, respectively, while the common subunit isreferred to as gammac. Although the common gamma chain enhancesligand binding in these three cytokine receptors, it has no capacityto bind these ligands on its own. There is evidence that the gammacchain is also a subunit of IL-13R.

NCBI and Uniprot Product Information

• NCBI GI #: 4557667
• NCBI GeneID: 3559
• NCBI Accession #: NP_000408.1
• NCBI GenBank Nucleotide #: NP_000408.1
• UniProt Accession #: P01589
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: interleukin-2 receptor subunit alpha isoform 1
• NCBI Official Synonym Full Names: interleukin 2 receptor subunit alpha
• NCBI Official Symbol: IL2RA
• NCBI Official Synonym Symbols: p55; CD25; IL2R; IMD41; TCGFR; IDDM10
• NCBI Protein Information: interleukin-2 receptor subunit alpha
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• Protein Family: Interleukin-2 receptor
• UniProt Gene Name: IL2RA
• UniProt Synonym Gene Names: IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_HUMAN

NCBI Description

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Uniprot Description

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: interleukin-2 receptor activity; drug binding; interleukin-2 binding

Biological Process: Notch signaling pathway; regulation of T cell homeostatic proliferation; apoptosis; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; cell proliferation; negative regulation of T cell proliferation; inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; positive regulation of T cell differentiation; negative regulation of inflammatory response; immune response; inflammatory response; activated T cell apoptosis

Disease: Interleukin 2 Receptor, Alpha, Deficiency Of; Diabetes Mellitus, Insulin-dependent, 10