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Western Blot (WB) (All lanes: Aspartate aminotransferase, cytoplasmic antibody at 2ug/ml+mouse brain tissue)

Rabbit anti-Human Aspartate aminotransferase Polyclonal Antibody | anti-GOT1 antibody

Aspartate aminotransferase, cytoplasmic Polyclonal Antibody

Gene Names
GOT1; AST1; cCAT; GIG18; cAspAT; ASTQTL1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Aspartate aminotransferase; Polyclonal Antibody; cytoplasmic Polyclonal Antibody; Glutamate oxaloacetate transaminase 1; Transaminase; GOT1; anti-GOT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total Aspartate aminotransferase, cytoplasmic polyclonal antibody.
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
413
Applicable Applications for anti-GOT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200
Immunogen
Recombinant Human Aspartate aminotransferase, cytoplasmic protein
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(All lanes: Aspartate aminotransferase, cytoplasmic antibody at 2ug/ml+mouse brain tissue)

Western Blot (WB) (All lanes: Aspartate aminotransferase, cytoplasmic antibody at 2ug/ml+mouse brain tissue)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embeddedmousebrainusing at dilution of 1:50.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embeddedmousebrainusing at dilution of 1:50.)
Related Product Information for anti-GOT1 antibody
Plays a key role in amino acid metabolism.
Product Categories/Family for anti-GOT1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,147 Da
NCBI Official Full Name
aspartate aminotransferase, cytoplasmic
NCBI Official Synonym Full Names
glutamic-oxaloacetic transaminase 1
NCBI Official Symbol
GOT1
NCBI Official Synonym Symbols
AST1; cCAT; GIG18; cAspAT; ASTQTL1
NCBI Protein Information
aspartate aminotransferase, cytoplasmic
UniProt Protein Name
Aspartate aminotransferase, cytoplasmic
UniProt Gene Name
GOT1
UniProt Synonym Gene Names
cAspAT; cCAT
UniProt Entry Name
AATC_HUMAN

Uniprot Description

GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; EC 2.6.1.3; Transferase

Chromosomal Location of Human Ortholog: 10q24.1-q25.1

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleus

Molecular Function: aspartate transaminase activity; cysteine transaminase activity; identical protein binding; pyridoxal phosphate binding

Biological Process: 2-oxoglutarate metabolic process; amino acid biosynthetic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; cellular response to insulin stimulus; gluconeogenesis; glutamate metabolic process; glycerol biosynthetic process; response to glucocorticoid stimulus

Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1

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Product Notes

The GOT1 got1 (Catalog #AAA9415502) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Aspartate aminotransferase, cytoplasmic Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartate aminotransferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:20 - 1:200. Researchers should empirically determine the suitability of the GOT1 got1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartate aminotransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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