Western Blot (WB)
(Western blot detection of Aspartate Aminotransferase in K562,Rat Brain,3T3,Hela cell lysates using Aspartate Aminotransferase Rabbit pAb(1:1000 diluted). Predicted band size:46kDa. Observed band size:46kDa.)
Rabbit Aspartate Aminotransferase Polyclonal Antibody | anti-GOT1 antibody
Aspartate Aminotransferase Rabbit pAb
IHC: 1:20-1:100
ICC/IF: 1:100
FC/FACS: 1:20-1:100
Western Blot (WB)
(Western blot detection of Aspartate Aminotransferase in K562,Rat Brain,3T3,Hela cell lysates using Aspartate Aminotransferase Rabbit pAb(1:1000 diluted). Predicted band size:46kDa. Observed band size:46kDa.)
Western Blot (WB)
(Western blot detection of Aspartate Aminotransferase in K562,Rat Brain,3T3,Hela cell lysates using Aspartate Aminotransferase Rabbit pAb(1:1000 diluted). Predicted band size:46kDa. Observed band size:46kDa.)
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
NCBI and Uniprot Product Information
NCBI Description
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
Uniprot Description
GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Amino Acid Metabolism - cysteine and methionine; Transferase; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.3
Chromosomal Location of Human Ortholog: 10q24.1-q25.1
Cellular Component: lysosome; cytoplasm; nerve terminal; nucleus; cytosol
Molecular Function: cysteine transaminase activity; phosphatidylserine decarboxylase activity; carboxylic acid binding; aspartate transaminase activity; pyridoxal phosphate binding
Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; response to glucocorticoid stimulus; glucose metabolic process; aspartate biosynthetic process; pathogenesis; gluconeogenesis; 2-oxoglutarate metabolic process; methionine salvage; cellular response to insulin stimulus; sulfur amino acid metabolic process; glycerol biosynthetic process; fatty acid homeostasis; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate; polyamine metabolic process
Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1
Research Articles on GOT1
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Product Notes
The GOT1 got1 (Catalog #AAA4751808) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Aspartate Aminotransferase Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartate Aminotransferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1000 IHC: 1:20-1:100 ICC/IF: 1:100 FC/FACS: 1:20-1:100. Researchers should empirically determine the suitability of the GOT1 got1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartate Aminotransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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