Rabbit anti-Human ARL3 Polyclonal Antibody | anti-ARL3 antibody

ARL3 Antibody (C-term)

Cat. Num. AAA9202025

• Gene Names: ARL3; ARFL3
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ARL3; Polyclonal Antibody; ARL3 Antibody (C-term); ADP-ribosylation factor-like protein 3; ARFL3; anti-ARL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ARL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the C-terminal region of human ARL3.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 144-173
• Sequence Length: 182

• Applicable Applications for anti-ARL3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-ARL3 in A2058 cell line lysate (35ug/lane).ARL3 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ARL3 (arrow) using rabbit polyclonal ARL3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARL3 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-ARL3 antibody

ADP-ribosylation factors (ARFs) are low molecular weight GTP-binding proteins belonging to the RAS superfamily. The predicted 182-amino acid ARL3 (ADP-ribosylation like factor) protein shares 97% amino acid identity with rat ARLl3 and 43% identity with human ARF1. Like the ARFs, ARL3 has a glycine at position 2, the site of N myristoylation, and lacks cysteine residues near the C terminus, which are found in other members of the RAS family. Northern blot analysis detected a 1-kb ARL3 transcript in all tissues tested, with highest expression in heart and lung, and lower expression in brain, liver, kidney, ovary, and testis. A 5.5-kb transcript was also detected in most tissues, with highest expression in brain. Immunoblot analysis detected ARL3 in human tumor cell lines but not in normal rodent cells. Although ARL3 binds GTP, it is devoid of activity in the cholera toxin-dependent ADP-ribosylation of Gs, and is therefore classified as an ARF-like protein.

Product Categories/Family for anti-ARL3 antibody

Cancer; Signal Transduction

References

Cavenagh, M.M., et al., J. Biol. Chem. 269(29):18937-18942 (1994).
Adams, M.D., et al., Nature 377 (6547 Suppl), 3-174 (1995).
Kim, H.S., Cytogenet. Cell Genet. 83 (3-4), 246 (1998).
Wistow, G., et al., Mol. Vis. 8, 196-204 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4757774
• NCBI GeneID: 403
• NCBI Accession #: NP_004302.1
• NCBI GenBank Nucleotide #: NM_004311.3
• UniProt Accession #: P36405; Q53X83; Q5JSM2; B2R6C7
• Molecular Weight: 20456
• NCBI Official Full Name: ADP-ribosylation factor-like protein 3
• NCBI Official Synonym Full Names: ADP-ribosylation factor-like 3
• NCBI Official Symbol: ARL3
• NCBI Official Synonym Symbols: ARFL3
• NCBI Protein Information: ADP-ribosylation factor-like protein 3
• UniProt Protein Name: ADP-ribosylation factor-like protein 3
• Protein Family: ADP-ribosylation factor-like protein
• UniProt Gene Name: ARL3
• UniProt Synonym Gene Names: ARFL3
• UniProt Entry Name: ARL3_HUMAN

NCBI Description

ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. [provided by RefSeq, Jul 2008]

Uniprot Description

ARL3: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase- activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit. Belongs to the small GTPase superfamily. Arf family.

Protein type: Cytoskeletal; G protein, monomeric, ARF; G protein, monomeric; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: Golgi membrane; Golgi apparatus; centrosome; spindle microtubule; cytoplasmic microtubule; midbody; nucleus; photoreceptor connecting cilium

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; microtubule binding; metal ion binding

Biological Process: photoreceptor cell development; protein transport; smoothened signaling pathway; metabolic process; small GTPase mediated signal transduction; organelle organization and biogenesis; intraflagellar transport; cytokinesis; post-Golgi vesicle-mediated transport; kidney development