Rabbit ARL3 Polyclonal Antibody | anti-ARL3 antibody

ARL3 Polyclonal Antibody

Cat. Num. AAA2528064

• Gene Names: ARL3; ARFL3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Antigen affinity purification
• Synonyms: ARL3; Polyclonal Antibody; ARL3 Polyclonal Antibody; ARFL3; anti-ARL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1.3mg/mL (varies by lot)
• Sequence Length: 182

• Applicable Applications for anti-ARL3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:200-1:1000
• Immunogen: Synthetic peptide of human ARL3
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Mouse brain tissue and 231 cell, A375 cell using ARL3 Polyclonal Antibody at dilution of 1:433)

Related Product Information for anti-ARL3 antibody

ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms.

NCBI and Uniprot Product Information

• NCBI GI #: 4757774
• NCBI GeneID: 403
• NCBI Accession #: NP_004302.1
• NCBI GenBank Nucleotide #: NM_004311.3
• Molecular Weight: 20,456 Da
• NCBI Official Full Name: ADP-ribosylation factor-like protein 3
• NCBI Official Synonym Full Names: ADP-ribosylation factor-like 3
• NCBI Official Symbol: ARL3
• NCBI Official Synonym Symbols: ARFL3
• NCBI Protein Information: ADP-ribosylation factor-like protein 3; ARF-like 3
• UniProt Protein Name: ADP-ribosylation factor-like protein 3
• Protein Family: ADP-ribosylation factor-like protein
• UniProt Gene Name: ARL3
• UniProt Synonym Gene Names: ARFL3
• UniProt Entry Name: ARL3_HUMAN

NCBI Description

ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. [provided by RefSeq, Jul 2008]

Uniprot Description

ARL3: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase- activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric, ARF; Cytoskeletal; Cell cycle regulation; G protein, monomeric

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: Golgi membrane; Golgi apparatus; centrosome; cytoplasmic microtubule; spindle microtubule; midbody; nucleus; photoreceptor connecting cilium

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; microtubule binding; metal ion binding

Biological Process: photoreceptor cell development; smoothened signaling pathway; protein transport; metabolic process; small GTPase mediated signal transduction; organelle organization and biogenesis; intraflagellar transport; cytokinesis; post-Golgi vesicle-mediated transport; kidney development