SDS-Page
ARL3 recombinant protein
ARL3, 1-182aa, Human, His tag, E Coli
Gene Names
ARL3; ARFL3
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
ARL3; 1-182aa; Human; His tag; E Coli; ADP-ribosylation factor-like protein 3; ARFL3; ARL3 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
Concentration
1.0 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMGLLSI LRKLKSAPDQ EVRILLLGLD NAGKTTLLKQ LASEDISHIT PTQGFNIKSV QSQGFKLNVW DIGGQRKIRP YWKNYFENTD ILIYVIDSAD RKRFEETGQE LAELLEEEKL SCVPVLIFAN KQDLLTAAPA SEIAEGLNLH TIRDRVWQIQ SCSALTGEGV QDGMNWVCKN VNAKKK
Sequence Length
182
Applicable Applications for ARL3 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for ARL3 recombinant protein
ARL3, also known as ARFL3, is a member of the ARF-like protein (ARL) subfamily of small GTPases. unlike ARFs, ARL3 does not activate the cholera toxin ADP-ribosyltranferase. This protein is ubiquitously expressed. It may play a role in maintaining rod photoreceptor cells and regulating microtubule-dependent processes. Knockdown of ARL3 leads to altered cell morphology, failure of cytokinesis, acetylation of alpha tubulin and an increase in binucleated cells. Recombinant human ARL3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for ARL3 recombinant protein
References
Bartolini F. et al. (2002) J Biol Chem. 277:14629-14634.; Hillig R.C. et al. (2001) Structure. 8:1239-1245.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23.0 kDa (206aa), confirmed by MALDI-TOF
NCBI Official Full Name
ADP-ribosylation factor-like protein 3
NCBI Official Synonym Full Names
ADP ribosylation factor like GTPase 3
NCBI Official Symbol
ARL3
NCBI Official Synonym Symbols
ARFL3
NCBI Protein Information
ADP-ribosylation factor-like protein 3
UniProt Protein Name
ADP-ribosylation factor-like protein 3
Protein Family
UniProt Gene Name
ARL3
UniProt Synonym Gene Names
ARFL3
NCBI Description
ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. [provided by RefSeq, Jul 2008]
Uniprot Description
Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit.
Research Articles on ARL3
Similar Products
Product Notes
The ARL3 arl3 (Catalog #AAA204765) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's ARL3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the ARL3 arl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMGLLSI LRKLKSAPDQ EVRILLLGLD NAGKTTLLKQ LASEDISHIT PTQGFNIKSV QSQGFKLNVW DIGGQRKIRP YWKNYFENTD ILIYVIDSAD RKRFEETGQE LAELLEEEKL SCVPVLIFAN KQDLLTAAPA SEIAEGLNLH TIRDRVWQIQ SCSALTGEGV QDGMNWVCKN VNAKKK. It is sometimes possible for the material contained within the vial of "ARL3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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