Rabbit Cholecystokinin A Receptor (CCKAR) ELISA Kit | CCKAR elisa kit

Rabbit Cholecystokinin A Receptor (CCKAR) ELISA Kit

Cat. Num. AAA2701216

• Gene Names: CCKAR; CCK-A; CCK1R; CCKRA; CCK1-R
• Reactivity: Rabbit
• Synonyms: Cholecystokinin A Receptor (CCKAR); Rabbit Cholecystokinin A Receptor (CCKAR) ELISA Kit; CCK-A; CCK1; CCKRA; CCK1R; Cholecystokinin Type 1 Receptor; CCKAR elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cholecystokinin A Receptor (CCKAR).; No significant cross-reactivity or interference between Cholecystokinin A Receptor (CCKAR) and analogues was observed.
• Sequence Length: 428

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.61ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholecystokinin A Receptor (CCKAR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholecystokinin A Receptor (CCKAR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCKAR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholecystokinin A Receptor (CCKAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholecystokinin A Receptor (CCKAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholecystokinin A Receptor (CCKAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholecystokinin A Receptor (CCKAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502607
• NCBI GeneID: 886
• NCBI Accession #: NP_000721.1
• NCBI GenBank Nucleotide #: NM_000730.2
• UniProt Accession #: P32238; B2R9Z5
• Molecular Weight: 47,841 Da
• NCBI Official Full Name: cholecystokinin receptor type A
• NCBI Official Synonym Full Names: cholecystokinin A receptor
• NCBI Official Symbol: CCKAR
• NCBI Official Synonym Symbols: CCK-A; CCK1R; CCKRA; CCK1-R
• NCBI Protein Information: cholecystokinin receptor type A
• UniProt Protein Name: Cholecystokinin receptor type A
• Protein Family: Cholecystokinin receptor
• UniProt Gene Name: CCKAR
• UniProt Synonym Gene Names: CCKRA; CCK-A receptor; CCK-AR; CCK1-R

NCBI Description

This gene encodes a G-protein coupled receptor that binds non-sulfated members of the cholecystokinin (CCK) family of peptide hormones. This receptor is a major physiologic mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach. In the central and peripheral nervous system this receptor regulates satiety and the release of beta-endorphin and dopamine. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.