Rabbit anti-Human, Mouse Apobec1 Polyclonal Antibody | anti-APOBEC1 antibody

Apobec1, NT (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide 1, ABEDP, CDAR1, HEPR, Apolipoprotein B mRNA Editing Enzyme)

Cat. Num. AAA623519

• Gene Names: APOBEC1; BEDP; HEPR; CDAR1; APOBEC-1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Apobec1; Polyclonal Antibody; NT (Apolipoprotein B mRNA Editing Enzyme; Catalytic Polypeptide 1; ABEDP; CDAR1; HEPR; Apolipoprotein B mRNA Editing Enzyme); Anti -Apobec1; anti-APOBEC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Apobec1. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-APOBEC1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human Apobec1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-Apobec1 N-term Pab is used in Western blot to detect Apobec in mouse small intestine tissue lysate.)

Western Blot (WB)

(Western blot analysis of anti-Apobec1 Antibody (N-term) in 293 cell line lysates (35ug/lane). Apobec1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-APOBEC1 antibody

APOBEC1 is involved in the production of apolipoprotein B (apoB)-48 from apoB-100. The gene spans 18 kb and contains five exons, all of which are translated. Alternative splicing produces a variant transcript that lacks exon 2 and encodes a novel 36aa peptide. The exon 2-skipped transcript accounts for approximately 50% of APOBEC1 mRNA in the adult small intestine and up to 90% of APOBEC1 mRNA in the developing gut. Exon 2-skipping may thus be a quantitatively important mechanism for regulating the expression of this gene in the gastrointestinal tract.

Product Categories/Family for anti-APOBEC1 antibody

Antibodies; Abs to Enzymes; Cytidine Deaminase (APOBEC)

NCBI and Uniprot Product Information

• NCBI GI #: 66365008
• NCBI GeneID: 339
• UniProt Accession #: Q4VB33; P41238
• Molecular Weight: 26,771 Da
• NCBI Official Full Name: APOBEC1 protein
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1
• NCBI Official Symbol: APOBEC1
• NCBI Official Synonym Symbols: BEDP; HEPR; CDAR1; APOBEC-1
• NCBI Protein Information: C->U-editing enzyme APOBEC-1; OTTHUMP00000180598; apolipoprotein B mRNA-editing enzyme 1; apolipoprotein B mRNA editing enzyme complex-1
• UniProt Protein Name: APOBEC1 protein
• Protein Family: APOBEC1 complementation factor
• UniProt Gene Name: APOBEC1
• UniProt Entry Name: Q4VB33_HUMAN

NCBI Description

This gene encodes a member of the cytidine deaminase enzyme family. The encoded protein forms a multiple-protein editing holoenzyme with APOBEC1 complementation factor (ACF) and APOBEC1 stimulating protein (ASP). This holoenzyme is involved in the editing of C-to-U nucleotide bases in apolipoprotein B and neurofibromatosis-1 mRNAs. [provided by RefSeq]

Uniprot Description

APOBEC1: apolipoprotein B mRNA editing enzyme. Responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. Expressed exclusively in the small intestine.

Protein type: Cell cycle regulation; EC 3.5.4.-; Hydrolase; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; zinc ion binding; RNA binding; AU-rich element binding; cytidine deaminase activity

Biological Process: response to drug; mRNA stabilization; response to osmotic stress; regulation of cell proliferation; RNA processing; response to ethanol; cytidine to uridine editing; cellular response to insulin stimulus; response to zinc ion; lipoprotein biosynthetic process; lipoprotein transport; mRNA modification; response to gamma radiation; gene expression; cytidine deamination; lipid metabolic process; response to calcium ion; mRNA processing; defense response to virus