Rabbit anti-Human, Mouse Apobec1 Polyclonal Antibody | anti-Apobec1 antibody

Apobec1, NT (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide 1, ABEDP, CDAR1, HEPR, Apolipoprotein B mRNA Editing Enzyme) (FITC)

Cat. Num. AAA6463492

• Gene Names: APOBEC1; BEDP; HEPR; CDAR1; APOBEC-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Apobec1; Polyclonal Antibody; NT (Apolipoprotein B mRNA Editing Enzyme; Catalytic Polypeptide 1; ABEDP; CDAR1; HEPR; Apolipoprotein B mRNA Editing Enzyme) (FITC); anti-Apobec1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Apobec1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Apobec1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human Apobec1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-Apobec1 N-term Pab is used in Western blot to detect Apobec in mouse small intestine tissue lysate.)

Western Blot (WB)

(Western blot analysis of anti-Apobec1 Antibody (N-term) in 293 cell line lysates (35ug/lane). Apobec1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-Apobec1 antibody

Antibodies; Enzymes; Cytidine Deaminase (APOBEC)

References

1. Blanc, V., et al., J. Biol. Chem. 278(42):41198-41204 (2003). 2. Chester, A., et al., EMBO J. 22(15):3971-3982 (2003). 3. Wedekind, J.E., et al., Trends Genet. 19(4):207-216 (2003). 4. Mukhopadhyay, D., et al., Am. J. Hum. Genet. 70(1):38-50 (2002). 5. Dance, G.S., et al., J. Biol. Chem. 277(15):12703-12709 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 61743957
• NCBI GeneID: 339
• NCBI Accession #: NP_001635
• NCBI GenBank Nucleotide #: NM_001644
• UniProt Accession #: P41238
• Molecular Weight: 28kDa
• NCBI Official Full Name: C->U-editing enzyme APOBEC-1 isoform a
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme catalytic subunit 1
• NCBI Official Symbol: APOBEC1
• NCBI Official Synonym Symbols: BEDP; HEPR; CDAR1; APOBEC-1
• NCBI Protein Information: C->U-editing enzyme APOBEC-1
• UniProt Protein Name: C->U-editing enzyme APOBEC-1
• Protein Family: APOBEC1 complementation factor
• UniProt Gene Name: APOBEC1
• UniProt Entry Name: ABEC1_HUMAN

NCBI Description

This gene encodes a member of the cytidine deaminase enzyme family. The encoded protein forms a multiple-protein editing holoenzyme with APOBEC1 complementation factor (ACF) and APOBEC1 stimulating protein (ASP). This holoenzyme is involved in the editing of C-to-U nucleotide bases in apolipoprotein B and neurofibromatosis-1 mRNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2015]

Uniprot Description

Function: Catalytic component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Ref.8

Cofactor: Zinc

By similarity.

Subunit structure: Homodimer. Part of the apolipoprotein B mRNA editing complex with A1CF. Found in a complex with CELF2/CUGBP2 and A1CF. Interacts with HNRPAB and SYNCRIP. Ref.6 Ref.7

Subcellular location: Cytoplasm Ref.9.

Tissue specificity: Expressed exclusively in the small intestine.

Sequence similarities: Belongs to the cytidine and deoxycytidylate deaminase family.