Rabbit anti-Human, mouse Apobec1 Polyclonal Antibody | anti-APOBEC1 antibody

Apobec1 Antibody (N-term)

Cat. Num. AAA9200910

• Gene Names: APOBEC1; BEDP; HEPR; CDAR1; APOBEC-1
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Apobec1; Polyclonal Antibody; Apobec1 Antibody (N-term); C->U-editing enzyme APOBEC-1; 354-; Apolipoprotein B mRNA-editing enzyme 1; HEPR; APOBEC1; anti-APOBEC1 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Apobec1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-36 amino acids from the N-terminal region of human Apobec1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 7-36
• Sequence Length: 236

• Applicable Applications for anti-APOBEC1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-Apobec1 N-term Pab is used in Western blot to detect Apobec in mouse small intestine tissue lysate.)

Western Blot (WB)

(Western blot analysis of anti-Apobec1 Antibody (N-term) in 293 cell line lysates (35ug/lane). Apobec1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-APOBEC1 antibody

APOBEC1 is involved in the production of apolipoprotein B (apoB)-48 from apoB-100. The gene spans 18 kb and contains five exons, all of which are translated. Alternative splicing produces a variant transcript that lacks exon 2 and encodes a novel 36-amino acid peptide. The exon 2-skipped transcript accounts for approximately 50% of APOBEC1 mRNA in the adult small intestine and up to 90% of APOBEC1 mRNA in the developing gut. Exon 2-skipping may thus be a quantitatively important mechanism for regulating the expression of this gene in the gastrointestinal tract.

References

Blanc, V., et al., J. Biol. Chem. 278(42):41198-41204 (2003).
Chester, A., et al., EMBO J. 22(15):3971-3982 (2003).
Wedekind, J.E., et al., Trends Genet. 19(4):207-216 (2003).
Mukhopadhyay, D., et al., Am. J. Hum. Genet. 70(1):38-50 (2002).
Dance, G.S., et al., J. Biol. Chem. 277(15):12703-12709 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 61743957
• NCBI GeneID: 339
• NCBI Accession #: NP_001635.2
• NCBI GenBank Nucleotide #: NM_001644.4
• UniProt Accession #: P41238; Q9UE64; Q9UM71
• Molecular Weight: 28192
• NCBI Official Full Name: C->U-editing enzyme APOBEC-1 isoform a
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1
• NCBI Official Symbol: APOBEC1
• NCBI Official Synonym Symbols: BEDP; HEPR; CDAR1; APOBEC-1
• NCBI Protein Information: C->U-editing enzyme APOBEC-1
• UniProt Protein Name: C->U-editing enzyme APOBEC-1
• Protein Family: APOBEC1 complementation factor
• UniProt Gene Name: APOBEC1
• UniProt Entry Name: ABEC1_HUMAN

NCBI Description

This gene encodes a member of the cytidine deaminase enzyme family. The encoded protein forms a multiple-protein editing holoenzyme with APOBEC1 complementation factor (ACF) and APOBEC1 stimulating protein (ASP). This holoenzyme is involved in the editing of C-to-U nucleotide bases in apolipoprotein B and neurofibromatosis-1 mRNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2015]

Uniprot Description

APOBEC1: apolipoprotein B mRNA editing enzyme. Responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. Expressed exclusively in the small intestine.

Protein type: RNA processing; Hydrolase; RNA-binding; Cell cycle regulation; EC 3.5.4.-

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; zinc ion binding; RNA binding; AU-rich element binding; cytidine deaminase activity

Biological Process: response to drug; mRNA stabilization; response to osmotic stress; regulation of cell proliferation; RNA processing; response to ethanol; cellular response to insulin stimulus; cytidine to uridine editing; response to zinc ion; lipoprotein transport; lipoprotein biosynthetic process; mRNA modification; response to gamma radiation; gene expression; cytidine deamination; lipid metabolic process; mRNA processing; response to calcium ion; defense response to virus