Chicken CR ELISA Kit | CR elisa kit
Chicken CR (Calretinin) ELISA Kit
Reactivity
Chicken
Synonyms
CR; Chicken CR (Calretinin) ELISA Kit; CR elisa kit
Reactivity
Chicken
Specificity
This kit recognizes natural and recombinant Chicken CR. No significant cross-reactivity or interference between Chicken CR and analogues was observed.
Sequence Length
271
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
7.813-500ng/mL
Sensitivity
Min: 4.688ng/mL; Max: 500ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CR elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken CR concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CR. You can calculate the concentration of CR in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CR. You can calculate the concentration of CR in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,405 Da
NCBI Official Full Name
calretinin
NCBI Official Synonym Full Names
calbindin 2
NCBI Official Symbol
Calb2
NCBI Protein Information
calretinin
UniProt Protein Name
Calretinin
Protein Family
UniProt Gene Name
Calb2
UniProt Synonym Gene Names
CR
UniProt Entry Name
CALB2_RAT
NCBI Description
displays increased mRNA expression following trimethyltin-induced neurodegeneration [RGD, Feb 2006]
Uniprot Description
CALB2: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Belongs to the calbindin family.
Protein type: Calcium-binding
Cellular Component: cytoplasm; gap junction; terminal button; nucleus
Molecular Function: calcium ion binding
Biological Process: cytosolic calcium ion homeostasis
