Rabbit anti-Human Apg12 Polyclonal Antibody | anti-ATG12 antibody

Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)

Cat. Num. AAA618240

• Gene Names: ATG12; APG12; FBR93; APG12L; HAPG12
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified immunoaffinity chromatography.
• Synonyms: Apg12; Polyclonal Antibody; Apg12 (Autophagy-related 12; 4931423H11Rik; A330058M13Rik; Atg12; Apg12L; APG12-like; Atg12L; Autophagy-related protein 12); Anti -Apg12 (Autophagy-related 12; anti-ATG12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total free at 16kD and Atg5 bound Atg12 protein at 53kD.
• Purity/Purification: Affinity Purified; Purified immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-ATG12 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunoprecipitation.; Dilution: Immunofluorescence: 1:100; Western Blot: 1:1000; Immunoprecipitation: 1:25
• Immunogen: Synthetic peptide corresponding to residues near the amino terminus of human Atg12 (KLH).
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from various cell lines using MBS618240 (Human Specific).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HCT-116 cells, untreated (left) or chloroquine-treated (right), using MBS618240 (Human Specific) (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)

Related Product Information for anti-ATG12 antibody

Autophagy is a catabolic process for the autophagosomic- lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Apg12 is covalently bound to Apg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Apg7 and the E2-like enzyme Apg10 (7,8).

Product Categories/Family for anti-ATG12 antibody

Antibodies; Abs to Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 290560746
• NCBI GeneID: 9140
• NCBI Accession #: NP_004698.3
• NCBI GenBank Nucleotide #: NM_004707.3
• UniProt Accession #: O94817; Q6PJV2
• Molecular Weight: 15,113 Da
• NCBI Official Full Name: ubiquitin-like protein ATG12
• NCBI Official Synonym Full Names: ATG12 autophagy related 12 homolog (S. cerevisiae)
• NCBI Official Symbol: ATG12
• NCBI Official Synonym Symbols: APG12; FBR93; APG12L; HAPG12
• NCBI Protein Information: ubiquitin-like protein ATG12; OTTHUMP00000159129; autophagy-related protein 12; Apg12 (autophagy, yeast) homolog
• UniProt Protein Name: Ubiquitin-like protein ATG12
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG12
• UniProt Synonym Gene Names: APG12; APG12L
• UniProt Entry Name: ATG12_HUMAN

NCBI Description

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

Uniprot Description

ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: pre-autophagosomal structure membrane

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: mitochondrion degradation; innate immune response; C-terminal protein lipidation; negative regulation of interferon type I production; cellular response to nitrogen starvation; autophagic vacuole formation