Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48 and 55kDa ATG12 protein using Rabbit Anti-ATG12 Polyclonal Antibody (SPC-607). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody (SPC-607) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~48 and 55kDa.)
Rabbit anti-Human ATG12 Polyclonal Antibody | anti-ATG12 antibody
Rabbit Anti-Human ATG12 Polyclonal
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48 and 55kDa ATG12 protein using Rabbit Anti-ATG12 Polyclonal Antibody (SPC-607). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody (SPC-607) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~48 and 55kDa.)
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48 and 55kDa ATG12 protein using Rabbit Anti-ATG12 Polyclonal Antibody (SPC-607). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody (SPC-607) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~48 and 55kDa.)
NCBI and Uniprot Product Information
NCBI Description
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
Uniprot Description
ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy; Ubiquitin-like modifier
Chromosomal Location of Human Ortholog: 5q21-q22
Cellular Component: cytosol; phagocytic vesicle membrane; pre-autophagosomal structure membrane
Molecular Function: APG8 conjugating enzyme activity; protein binding
Biological Process: autophagic vacuole formation; C-terminal protein lipidation; innate immune response; macroautophagy; macromitophagy; mitochondrion degradation; negative regulation of interferon type I production
Research Articles on ATG12
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Product Notes
The ATG12 atg12 (Catalog #AAA807754) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human ATG12 Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATG12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:1000. Researchers should empirically determine the suitability of the ATG12 atg12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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