Rabbit APC5 Polyclonal Antibody | anti-ANAPC5 antibody

APC5 Antibody

Cat. Num. AAA151299

• Gene Names: ANAPC5; APC5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: APC5 Antibody is affinity chromatography purified via peptide column.
• Synonyms: APC5; Polyclonal Antibody; APC5 Antibody; Anaphase-promoting complex subunit 5; Cyclosome subunit 5; anaphase promoting complex subunit 5; anti-ANAPC5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: APC5 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 755

• Applicable Applications for anti-ANAPC5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: APC5 antibody can be used for detection of APC5 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: APC5 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC5.
• Buffer: APC5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: APC5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of APC5 in human kidney tissue lysate with APC5 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of APC5 in rat kidney tissue with APC5 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of APC5 in rat kidney tissue with APC5 antibody at 20 μg/mL.)

Related Product Information for anti-ANAPC5 antibody

APC5 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC5, it is thought that APC5 associates with other APC/C components APC1, APC4, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.

NCBI and Uniprot Product Information

• NCBI GI #: 37537861
• NCBI GeneID: 51433
• UniProt Accession #: Q9UJX4; Q8N4H7; Q9BQD4; E9PFB2
• Molecular Weight: 72,526 Da
• NCBI Official Full Name: Anaphase-promoting complex subunit 5
• NCBI Official Synonym Full Names: anaphase promoting complex subunit 5
• NCBI Official Symbol: ANAPC5
• NCBI Official Synonym Symbols: APC5
• NCBI Protein Information: anaphase-promoting complex subunit 5; cyclosome subunit 5
• UniProt Protein Name: Anaphase-promoting complex subunit 5
• UniProt Gene Name: ANAPC5
• UniProt Synonym Gene Names: APC5; APC5
• UniProt Entry Name: APC5_HUMAN

NCBI Description

This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]

Uniprot Description

APC5: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; anaphase-promoting complex; nucleus; cytosol

Molecular Function: protein phosphatase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle