Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, Jurkat cells and COLO205 cells, using ANAPC5 antibody.)
Rabbit anti-Human ANAPC5 Polyclonal Antibody | anti-ANAPC5 antibody
ANAPC5 Antibody
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, Jurkat cells and COLO205 cells, using ANAPC5 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, Jurkat cells and COLO205 cells, using ANAPC5 antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]
Uniprot Description
APC5: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC5 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: nucleoplasm; anaphase-promoting complex; nucleus; cytosol
Molecular Function: protein phosphatase binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
Research Articles on ANAPC5
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Product Notes
The ANAPC5 anapc5 (Catalog #AAA9410364) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANAPC5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ANAPC5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:3000. Researchers should empirically determine the suitability of the ANAPC5 anapc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANAPC5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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