Rabbit anti-Human, Mouse Angiotensin Converting Enzyme 2, IN1 Polyclonal Antibody | anti-Ace2 antibody

Angiotensin Converting Enzyme 2, IN1 (ACE-2, ACEH)

Cat. Num. AAA611394

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Angiotensin Converting Enzyme 2; IN1; Polyclonal Antibody; IN1 (ACE-2; ACEH); Anti -Angiotensin Converting Enzyme 2; anti-Ace2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes ACE2 (IN1) and has no cross response to ACE1. Species Crossreactivity: Human, mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

• Applicable Applications for anti-Ace2 antibody: Western Blot (WB)
• Application Notes: Can be used for the detection of ACE2 by Western blot at 0.5 to 2ug/ml. Human kidney or human testis cell lysate can be used as positive control and a 90 kD band can be detected.
• Immunogen: Synthetic peptide corresponding to amino acids 192 to 208 of human ACE2 (Genbank accession NP_068576)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of ACE2 in human kidney lysate with anti-ACE2 (IN1) at 0.5 (lane A), 1 (lane B), and 2 (lane C) ug/ml, respectively.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of MBS611394.)

Related Product Information for anti-Ace2 antibody

Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology (1-2). In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus (3). The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function (4).

Product Categories/Family for anti-Ace2 antibody

Antibodies; Abs to Enzymes; Angiotensin Converting (ACE)

NCBI and Uniprot Product Information

• NCBI GI #: 58865588
• NCBI GeneID: 302668
• NCBI Accession #: NP_001012006.1
• NCBI GenBank Nucleotide #: NM_001012006.1
• UniProt Accession #: Q5EGZ1
• Molecular Weight: 92,491 Da
• NCBI Official Full Name: angiotensin-converting enzyme 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• NCBI Official Symbol: Ace2
• NCBI Protein Information: angiotensin-converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; renal angiotensin-converting enzyme 2; anigotensin-converting enzyme-related carboxypeptidase
• UniProt Protein Name: Angiotensin-converting enzyme 2
• Protein Family: Angiotensin-converting enzyme
• UniProt Gene Name: Ace2
• UniProt Entry Name: ACE2_RAT

NCBI Description

enzyme peptidyl-dipeptidase; mouse homolog has a role in regulation of cardiac contractility and regulating Angiotensin 1 and 2 levels [RGD]

Uniprot Description

Function: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. Ref.2

Catalytic activity: Angiotensin II + H2O = angiotensin-1-7 + L-phenylalanine.

Cofactor: Binds 1 zinc ion per subunit

By similarity.Binds 1 chloride ion per subunit

Enzyme regulation: Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril, enalaprilat. Ref.3

Subcellular location: Processed angiotensin-converting enzyme 2: Secreted

By similarity Ref.3. Cell membrane; Single-pass type I membrane protein

By similarity Ref.3.

Tissue specificity: Expressed in heart, kidney and forebrain. In testis, expression is restricted to Leydig cells. In heart, expressed in endothelial cells from small and large arteries, arterial smooth muscle cells, and myocytes (at protein level). Ubiquitously expressed, with highest levels in ileum, bladder and lung. Ref.3 Ref.4 Ref.5

Induction: Down-regulated in hypertensive animals. Up-regulated after myocardial infarction. Ref.2 Ref.3 Ref.4

Post-translational modification: Glycosylated. Ref.3

Miscellaneous: In contrast to its human and palm-civet orthologs, does not interact with SARS-CoV spike glycoprotein.

Sequence similarities: Belongs to the peptidase M2 family.