ACE2 / Angiotensin-Converting Enzyme 2 Recombinant Protein | ACE2 recombinant protein

Recombinant Rat ACE2 / Angiotensin-Converting Enzyme 2 Protein (His tag)

Cat. Num. AAA2547490

• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: ACE2 / Angiotensin-Converting Enzyme 2; Recombinant Rat ACE2 / Angiotensin-Converting Enzyme 2 Protein (His tag); ACE2; ACE2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 805

• Application Notes: The recombinant rat ACE2 comprises 734 amino acids and predicts a molecular mass of 85 kDa. The apparent molecular mass of the rat ACE2 is approximately 105-115 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 18
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ACE2 recombinant protein

Background: Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme, an homolog of ACE, hydrolyzes angiotensin (Ang) I to produce Ang- (1-9), which is subsequently converted into Ang- (1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang- (1-7) more efficiently than its catalysis of Ang- (1-9) by cleavage of Pro (7)-Phe (8) bound in Ang II. Thus, the major biologically active product of ACE2 is Ang- (1-7), which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension, cardiac function, heart function and diabetes, and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis.

Description: A DNA sequence encoding the rat ACE2 (Q5EGZ1) extracellular domain (Met 1-Thr 740) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 58865588
• NCBI GeneID: 302668
• NCBI Accession #: NP_001012006.1
• NCBI GenBank Nucleotide #: NM_001012006.1
• UniProt Accession #: Q5EGZ1
• Molecular Weight: 92,491 Da
• NCBI Official Full Name: angiotensin-converting enzyme 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme 2
• NCBI Official Symbol: Ace2
• NCBI Protein Information: angiotensin-converting enzyme 2
• UniProt Protein Name: Angiotensin-converting enzyme 2
• Protein Family: Angiotensin-converting enzyme
• UniProt Gene Name: Ace2

NCBI Description

enzyme peptidyl-dipeptidase; mouse homolog has a role in regulation of cardiac contractility and regulating Angiotensin 1 and 2 levels [RGD, Feb 2006]

Uniprot Description

Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function.