Testing Data
Rabbit anti-Human Angiopoietin 2 Polyclonal Antibody | anti-ANGPT2 antibody
Anti-Mouse Angiopoietin 2 Antibody
Gene Names
ANGPT2; ang-2
Reactivity
Human
Applications
Western Blot
Purity
Purified
Synonyms
Angiopoietin 2; Polyclonal Antibody; Anti-Mouse Angiopoietin 2 Antibody; Mouse Angiopoietin 2; anti-ANGPT2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antiserum is directed against mouse angiopoietin 2 and shows no reactivity with angiopoietin 1 on mouse derived proteins. A 75 kDa band is detected when assayed by immunoblot. This reagent cross reacts with human angiopoietin 2. Partially cross reactivity is noted with human angiopoietin 1.
Purity/Purification
Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
192
Applicable Applications for anti-ANGPT2 antibody
Western Blot (WB)
Application Notes
Suitable for western blotting and other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in very clean and strong signal.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide N-N-F-R-K-S-V-D-S-T-G-R-R-Q-Y-Q-V-Q-N-G-P corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 2 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-ANGPT2 antibody
Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus. Anti-Ang 1 and anti-Ang 2 antibodies recognize a band above 55 kDa. Both antibodies show banding at 75 kDa which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
angiopoietin 2, partial
NCBI Official Symbol
ANGPT2
NCBI Official Synonym Symbols
ang-2
NCBI Protein Information
angiopoietin-2
Protein Family
Similar Products
Product Notes
The ANGPT2 (Catalog #AAA4156799) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse Angiopoietin 2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Angiopoietin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for western blotting and other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in very clean and strong signal. Researchers should empirically determine the suitability of the ANGPT2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiopoietin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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