Mouse anti-Human NOTCH3 Monoclonal Antibody | anti-NOTCH3 antibody

NOTCH3, CT (Notch homolog 3, Drosophila, hN3, CASIL, CADASIL) (PE)

Cat. Num. AAA6121727

• Gene Names: NOTCH3; IMF2; LMNS; CASIL; CADASIL; CADASIL1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: NOTCH3; Monoclonal Antibody; CT (Notch homolog 3; Drosophila; hN3; CASIL; CADASIL) (PE); anti-NOTCH3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 487CT6.9.2
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NOTCH3 antibody: FLISA, Western Blot (WB)
• Application Notes: FLISA: 1:1,000; WB: 1:100-250; Applications are based on unconjugated antibody.
• Immunogen: NOTCH3 Mab is generated from mice immunized with a KLH conjugated synthetic peptide selected from the C-term region of human NOTCH3.
• Conjugate: PE
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze R-Phycoerythrin conjugates. Light sensitive.

Western Blot (WB)

(NOTCH3 Antibody (Q2306) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NOTCH3 antibody detected the NOTCH3 protein (arrow).)

Related Product Information for anti-NOTCH3 antibody

This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq].

Product Categories/Family for anti-NOTCH3 antibody

Antibodies; Notch Proteins

References

Liu, H., et al. Circ. Res. 107(7):860-870(2010) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Menon, S., et al. Cephalalgia (2010) In press Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010)

NCBI and Uniprot Product Information

• NCBI GI #: 134244285
• NCBI GeneID: 4854
• NCBI Accession #: NP_000426.2
• NCBI GenBank Nucleotide #: NM_000435.2
• UniProt Accession #: Q9UM47; Q9UEB3; Q9UPL3; Q9Y6L8
• Molecular Weight: 243,631 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 3
• NCBI Official Synonym Full Names: notch 3
• NCBI Official Symbol: NOTCH3
• NCBI Official Synonym Symbols: IMF2; LMNS; CASIL; CADASIL; CADASIL1
• NCBI Protein Information: neurogenic locus notch homolog protein 3
• UniProt Protein Name: Neurogenic locus notch homolog protein 3
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: NOTCH3
• UniProt Synonym Gene Names: Notch 3
• UniProt Entry Name: NOTC3_HUMAN

NCBI Description

This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008]

Uniprot Description

NOTCH3: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH3. Interacts with PSMA1. Interacts with HIF1AN. Ubiquitously expressed in fetal and adult tissues. Belongs to the NOTCH family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2-p13.1

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cytoplasm; integral to membrane; plasma membrane; extracellular region; cytosol; receptor complex; actin cytoskeleton

Molecular Function: protein binding; enzyme binding; calcium ion binding

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; forebrain development; neuron fate commitment; Notch receptor processing; gene expression

Disease: Cerebral Arteriopathy, Autosomal Dominant, With Subcortical Infarcts And Leukoencephalopathy; Lateral Meningocele Syndrome; Myofibromatosis, Infantile, 2