Human angiopoietin 2 ELISA Kit | ANGPT2 elisa kit

Human Angiopoietin 2, ANG-2 ELISA Kit

Cat. Num. AAA702275

• Gene Names: ANGPT2; ANG2; AGPT2
• Reactivity: Human
• Synonyms: angiopoietin 2; Human Angiopoietin 2; ANG-2 ELISA Kit; AGPT2; ANG2; Tie2-ligand; angiopoietin-2; angiopoietin-2B; angiopoietin-2a; ANGPT2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ANGPT2. No significant cross-reactivity or interference between Human ANGPT2 and analogues was observed.
• Sequence Length: 495

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 1.25 ng/ml-80 ng/ml
• Sensitivity: 0.31 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Angiogenesis
• Protein Biological Process 3: Angiogenesis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANGPT2 elisa kit

Introduction: Angiopoietin-2 (Ang-2) is one of the critical regulators of tumor angiogenesis. Human and mouse Ang-2 share 85% amino acid identity and Ang-2 is approximately 60% identical to Ang-1. Ang-2 contains an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. Located at the N-terminus are stretches of hydrophobic residues typical of secretory signal sequences. Although likely playing different functional roles, both Ang-1 and Ang-2 are ligands for the endothelial cell receptor tyrosine kinase Tie-2. The regulation of Tie-2 activity by Ang-2 is complex, leading to inhibition in certain cell types or conditions, and activation in others. In vitro studies demonstrated that Ang-1, but not Ang-2, could stimulate Tie-2 tyrosine phosphorylation and suggested that Ang-2 could act as a competitive inhibitor of Ang-1 signaling. Consistent with its role as an Ang-1/Tie-2 inhibitor, transgenic overexpression of Ang-2 leads to disruption of embryonic blood vessel formation, a phenotype similar to that of Tie-2 knockouts. In contrast, altering incubation times or elevating Ang-2 concentrations, can under some circumstances, result in Tie-2 activation in HUVECs. It has also been proposed that Ang-2 may play a pro-angiogenic role by mediating destabilizing interactions between endothelial and perivascular cells, enhancing the effects of pro-angiogenic proteins including vascular endothelial growth factor (VEGF). Alone, Ang-2 promotes endothelial cell death and vessel regression, but can act in synergy with VEGF to promote new vessel formation. Ang-2 has been implicated in cancer development due to its role in angiogenesis. Ang-2-mediated destabilization of mature vessel structures accompanied by the presence of VEGF, may contribute to the angiogenesis associated with tumor tissues. Several studies have examined Ang-2 expression in an assortment of human cancers. Ang-2 is elevated in colon carcinoma, gastric carcinoma, glioblastoma, glioma, hepatocellular carcinoma, neuroblastoma and non-small cell lung cancer. Furthermore, Ang-2 levels appear to correlate with advancing tumor stage progression and poor prognosis.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANG-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ANG-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ANG-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ANG-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 169646749
• NCBI GeneID: 285
• NCBI Accession #: NP_001112359.1
• NCBI GenBank Nucleotide #: NM_001118887.1
• UniProt Accession #: O15123; Q9NRR7; Q9P2Y7; A0AV38; A8K205; B7ZLM7
• Molecular Weight: 56,919 Da
• NCBI Official Full Name: angiopoietin-2 isoform b
• NCBI Official Synonym Full Names: angiopoietin 2
• NCBI Official Symbol: ANGPT2
• NCBI Official Synonym Symbols: ANG2; AGPT2
• NCBI Protein Information: angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a
• UniProt Protein Name: Angiopoietin-2
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT2
• UniProt Synonym Gene Names: ANG-2
• UniProt Entry Name: ANGP2_HUMAN

NCBI Description

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.4 PublicationsManual assertion based on experiment in:Ref.1