Goat anti-Human Angiogenin Polyclonal Antibody

Angiogenin

Cat. Num. AAA613693

• Reactivity: Human
• Applications: ELISA, Western Blot, Neutralization Assay
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: Angiogenin; Polyclonal Antibody; Anti -Angiogenin; anti-Angiogenin antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Angiogenin at ~14kD.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a liquid in 20mM Tris, pH 7.4, 0.1% BSA.

• Applicable Applications for anti-Angiogenin antibody: ELISA (EL/EIA), Western Blot (WB), Neutralization
• Application Notes: Suitable for use in Western Blot, ELISA and Neutralization.; Dilution: Western Blot: 1-5ug/ml using ECL.; ELISA: 0.5-2.0ug antibody and 10-50ng of control antigen/well.; Neutralization: 100-200ug IgG neutralizes 50% activity of 1ug of Angiogenin. Neutralizes biological activity of recombinant human Angiogenin as measured by measurement of ribonucleolytic activity using tRNA.; Optimal dilution determined by the researcher.
• Immunogen: Human Angiogenin protein expressed in E. coli.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Angiogenin antibody

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis. Angiogenin is a single polypeptide chain of 123aa (mol wt ~14kD) secreted by tumor cells. It is a potent inhibitor of neovascularization. It specifically binds to endothelial cells and elicits second messenger systems. It has ribonucleolytic activity and is 33% identical to pancreatic RNAse A.

Product Categories/Family for anti-Angiogenin antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 178250
• NCBI Official Full Name: angiogenin
• Protein Family: Angiogenin

Uniprot Description

ANG: May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. Belongs to the pancreatic ribonuclease family.

Protein type: Secreted, signal peptide; RNA-binding; Nucleolus; Motility/polarity/chemotaxis; Secreted; Ribonuclease; Extracellular matrix; Cell cycle regulation; EC 3.1.27.-

Chromosomal Location of Human Ortholog: 14q11.1-q11.2

Cellular Component: angiogenin-PRI complex; extracellular space; growth cone; cell soma; nucleolus; basal lamina; nucleus

Molecular Function: heparin binding; rRNA binding; protein binding; copper ion binding; DNA binding; endonuclease activity; actin binding; peptide binding; ribonuclease activity; receptor binding

Biological Process: cell migration; diacylglycerol biosynthetic process; negative regulation of smooth muscle cell proliferation; activation of protein kinase B; response to hormone stimulus; activation of phospholipase A2; oocyte maturation; rRNA transcription; ovarian follicle development; homeostatic process; positive regulation of protein secretion; actin filament polymerization; phospholipase C activation; negative regulation of translation; response to hypoxia; cell communication; positive regulation of endothelial cell proliferation; angiogenesis; positive regulation of phosphorylation; placenta development

Disease: Amyotrophic Lateral Sclerosis 9