Mouse anti-Human UCHL1 Monoclonal Antibody | anti-UCHL1 antibody

UCHL1 (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, UCH-L1, Neuron Cytoplasmic Protein 9.5, PGP 9.5, PGP9.5, Ubiquitin Thioesterase L1) (PE)

Cat. Num. AAA6160960

• Gene Names: UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UCHL1; Monoclonal Antibody; UCHL1 (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1; UCH-L1; Neuron Cytoplasmic Protein 9.5; PGP 9.5; PGP9.5; Ubiquitin Thioesterase L1) (PE); EC=3.4.19.12; EC=6.-.-.-; PARK5; PGP95; anti-UCHL1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1B8-4D2
• Specificity: Recognizes human UCHL1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-UCHL1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 1-10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-223 from human UCHL1 (AAH00332) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(UCHL1 monoclonal antibody, Western Blot analysis of UCHL1 expression in IMR-32.)

Western Blot (WB)

(Western Blot analysis of UCHL1 expression in transfected 293T cell line by UCHL1 monoclonal antibody. Lane 1: UCHL1 transfected lysate (Predicted MW: 24.8kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to UCHL1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1-10ug/ml])

Testing Data

(Detection limit for recombinant GST tagged UCHL1 is ~1ng/ml as a capture antibody.)

Product Categories/Family for anti-UCHL1 antibody

Antibodies; Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 33875314
• NCBI GeneID: 7345
• Molecular Weight: 24,824 Da
• NCBI Official Full Name: Homo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), mRNA
• NCBI Official Synonym Full Names: ubiquitin C-terminal hydrolase L1
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1
• Protein Family: Ubiquitin carboxyl-terminal hydrolase

NCBI Description

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]