Mouse anti-Human ANG Monoclonal Antibody | anti-ANG antibody

ANG (Angiogenin, Ribonuclease 5, RNase 5, RNASE5) (PE)

Cat. Num. AAA6156504

• Gene Names: ANG; ALS9; RAA1; HEL168; RNASE4; RNASE5
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ANG; Monoclonal Antibody; ANG (Angiogenin; Ribonuclease 5; RNase 5; RNASE5) (PE); anti-ANG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2A7
• Specificity: Recognizes human ANG.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ANG antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa25-147 from human ANG (AAH54880) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: QDNSRYTHFLTQHYDAKPQGRDDRYCESIMRRRGLTSPCKDINTFIHGNKRSIKAICENKNGNPHRENLRISKSSFQVTTCKLHGGSPWPPCQYRATAGFRNVVVACENGLPVHLDQSIFRRP
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (39.27kD).)

Western Blot (WB)

(Western Blot analysis of ANG expression in transfected 293T cell line by ANG monoclonal antibody. Lane 1: ANG transfected lysate (16.6kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-ANG antibody

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis. Angiogenin is a single polypeptide chain of 123aa (mol wt ~14kD) secreted by tumor cells. It is a potent inhibitor of neovascularization. It specifically binds to endothelial cells and elicits second messenger systems. It has ribonucleolytic activity and is 33% identical to pancreatic RNAse A.

Product Categories/Family for anti-ANG antibody

Antibodies; Enzymes; Ribonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 33392769
• NCBI GeneID: 283
• Molecular Weight: 16,550 Da
• NCBI Official Full Name: Homo sapiens angiogenin, ribonuclease, RNase A family, 5, mRNA
• NCBI Official Synonym Full Names: angiogenin
• NCBI Official Symbol: ANG
• NCBI Official Synonym Symbols: ALS9; RAA1; HEL168; RNASE4; RNASE5
• NCBI Protein Information: angiogenin
• Protein Family: Angiogenin

NCBI Description

The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]