Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-7: 231, hepg2, hela, Jurkat, NIH/3T3, K562 and PC3 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)
Rabbit anti-Human, Mouse PRPF19 Polyclonal Antibody | anti-PRPF19 antibody
PRPF19 Antibody
Immunohistochemistry: 1:10-1:50
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-7: 231, hepg2, hela, Jurkat, NIH/3T3, K562 and PC3 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-7: 231, hepg2, hela, Jurkat, NIH/3T3, K562 and PC3 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20. (Original magnification: ¡200))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20. (Original magnification: ¡200))
NCBI and Uniprot Product Information
Uniprot Description
PRPF19: Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Belongs to the WD repeat PRP19 family.
Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; RNA splicing; Spliceosome; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 11q12.2
Cellular Component: cytoplasm; DNA replication factor A complex; membrane; nuclear speck; nucleoplasm; nucleus
Molecular Function: identical protein binding; protein binding
Biological Process: assembly of spliceosomal tri-snRNP; double-strand break repair via nonhomologous end joining; generation of catalytic spliceosome for first transesterification step; nuclear mRNA splicing, via spliceosome; proteasomal protein catabolic process; protein polyubiquitination; spliceosome assembly; transcription-coupled nucleotide-excision repair
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Product Notes
The PRPF19 prpf19 (Catalog #AAA9415735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRPF19 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRPF19 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the PRPF19 prpf19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRPF19, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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