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SDS-Page

Adenylate Cyclase 7 Recombinant Protein | ADCY7 recombinant protein

Recombinant Adenylate Cyclase 7 (ADCY7)

Gene Names
ADCY7; AC7
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Adenylate Cyclase 7; Recombinant Adenylate Cyclase 7 (ADCY7); ADCY7 recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
6.2 (varies by lot)
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- FYITL LTLSRQIDYY CRLDCLWKKK FKKEHEEFET MENVNRLLLE NVLPAHVAAH FIGDKLNEDW YHQSYDCVCV MFASVPDFKV FYTECDVNKE GLECLRLLNE IIADFDELLL KPKFSGVEKI KTIGSTYMAA AGLSVASGHE NQELERQHAH IGVMVEFSIA LMSKLDGINR HSFNSFRLRV GINHGPVIAGVIGARKPQYD IWGNTVNVAS RMESTGELGK IQVTEETCTI LQGLGYSCEC RG
Sequence Length
1080
Applicable Applications for ADCY7 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
31.9kDa
Accurate Molecular Mass (KD)
32kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Homo sapiens (Human)
Fragment
Phe806~Gly1052
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
113
Molecular Weight
120,308 Da
NCBI Official Full Name
Adenylate cyclase 7
NCBI Official Synonym Full Names
adenylate cyclase 7
NCBI Official Symbol
ADCY7
NCBI Official Synonym Symbols
AC7
NCBI Protein Information
adenylate cyclase type 7
UniProt Protein Name
Adenylate cyclase type 7
Protein Family
UniProt Gene Name
ADCY7
UniProt Synonym Gene Names
KIAA0037
UniProt Entry Name
ADCY7_HUMAN

NCBI Description

This gene encodes a membrane-bound adenylate cyclase that catalyses the formation of cyclic AMP from ATP and is inhibitable by calcium. The product of this gene is a member of the adenylyl cyclase class-4/guanylyl cyclase enzyme family that is characterized by the presence of twelve membrane-spanning domains in its sequences. Several transcript variants have been observed for this gene, but the full-length natures of only two have been determined so far. [provided by RefSeq, Oct 2013]

Uniprot Description

ADCY7: This is a membrane-bound, calcium-inhibitable adenylyl cyclase. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Protein type: EC 4.6.1.1; Lyase; Transporter, aquaporin family; Membrane protein, integral; Adenylyl cyclase; Transporter; Nucleotide Metabolism - purine; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: plasma membrane; integral to membrane

Molecular Function: metal ion binding; ATP binding; adenylate cyclase activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; maternal process involved in pregnancy; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; signal transduction; G-protein signaling, adenylate cyclase activating pathway; synaptic transmission; phospholipase C activation; positive regulation of cAMP biosynthetic process; energy reserve metabolic process; renal water homeostasis; innate immune response; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; transmembrane transport

Research Articles on ADCY7

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Product Notes

The ADCY7 adcy7 (Catalog #AAA2030827) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Adenylate Cyclase 7 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the ADCY7 adcy7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- FYITL LTLSRQIDYY CRLDCLWKKK FKKEHEEFET MENVNRLLLE NVLPAHVAAH FIGDKLNEDW YHQSYDCVCV MFASVPDFKV FYTECDVNKE GLECLRLLNE IIADFDELLL KPKFSGVEKI KTIGSTYMAA AGLSVASGHE NQELERQHAH IGVMVEFSIA LMSKLDGINR HSFNSFRLRV GINHGPVIAG VIGARKPQYD IWGNTVNVAS RMESTGELGK IQVTEETCTI LQGLGYSCEC RG. It is sometimes possible for the material contained within the vial of "Adenylate Cyclase 7, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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