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Western Blot (WB)
(Western blot analysis of 30 ug whole cell lysate (A:A431; B:MOLT4) using a 7.5% SDS PAGE gel and AMPK alpha 2 antibody at a dilution of 1:1000)
Rabbit AMPK Alpha 2 Polyclonal Antibody | anti-AMPK2 antibody
AMPK Alpha 2 Antibody
Gene Names
PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
AMPK Alpha 2; Polyclonal Antibody; AMPK Alpha 2 Antibody; Affinity Purified Rabbit Polyclonal AMPK alpha 2 Antibody; Polyclonal AMPK alpha 2 antibody; Anti-AMPK alpha 2 antibody; AMPK2 antibody; AMPK alpha 2 antibody; PRKAA2 antibody; AMPK alpha 2; AMPK-alpha-2 chain antibody; PRKAA antibody; AMPK alpha -2 antibody; AMPK antibody; 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody; 5'-AMP-activated protein kinase catalytic alpha-2 chain antibody; AMPK alpha -2; anti-AMPK2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.55 mg/ml (varies by lot)
Sequence Length
552
Applicable Applications for anti-AMPK2 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
ICC: 1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Immunogen
AMPK alpha 2 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 29 and 510 of AMPK alpha 2
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 30 ug whole cell lysate (A:A431; B:MOLT4) using a 7.5% SDS PAGE gel and AMPK alpha 2 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug whole cell lysate (A:A431; B:MOLT4) using a 7.5% SDS PAGE gel and AMPK alpha 2 antibody at a dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded A549 xenograft using AMPK alpha 2 antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded A549 xenograft using AMPK alpha 2 antibody at a dilution of 1:500)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 7.5% SDS PAGE gel and AMPK alpha 2 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 7.5% SDS PAGE gel and AMPK alpha 2 antibody at a dilution of 1:1000)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using AMPK alpha 2 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using AMPK alpha 2 antibody at 1:200 dilution.)
Related Product Information for anti-AMPK2 antibody
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Product Categories/Family for anti-AMPK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
62 kDa
NCBI Official Full Name
AMP-activated protein kinase alpha 2 catalytic subunit
NCBI Official Synonym Full Names
protein kinase AMP-activated catalytic subunit alpha 2
NCBI Official Symbol
PRKAA2
NCBI Official Synonym Symbols
AMPK; AMPK2; PRKAA; AMPKa2
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-2
NCBI Description
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]
Research Articles on AMPK2
Similar Products
Product Notes
The AMPK2 (Catalog #AAA5311898) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AMPK Alpha 2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:500 ICC: 1:100-1:500 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the AMPK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPK Alpha 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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