AMPK alpha2 Protein Recombinant Protein | AMPKalpha2 recombinant protein

Recombinant Rat AMPK alpha2 Protein (His Tag)

Cat. Num. AAA2569431

• Gene Names: Prkaa2; Ampk; Ampka2
• Purity: >85% as determined by reducing SDS-PAGE
• Synonyms: AMPK alpha2 Protein; Recombinant Rat AMPK alpha2 Protein (His Tag); 5'-AMP-activated protein kinase catalytic subunit alpha-2; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMPK alpha 2 chain; AMPK subunit alpha-2; AMPK2; AMPKa2; AMPKalpha2; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; PRKAA; PRKAA2; Protein kinase AMP activated alpha 2 catalytic subunit; Protein kinase AMP activated catalytic subunit alpha 2; AMPKalpha2 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by reducing SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris,1mM EDTA, 25% glycerol, pH8.0
• Sequence: Tyr16-Val195

• Species: Rat
• Tag: N-His
• Endotoxin: Not tested.
• Protein Construction: A DNA sequence encoding the Rat AMPK alpha2 (Q09137-1)(Tyr16-Val195) was expressed with a polyhistidine tag at the N-terminus.
• Preparation and Storage: Store at < -20 degree C, stable for 6 months. Please minimize freeze-thaw cycles.; This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20 degree C.

SDS-Page

Related Product Information for AMPKalpha2 recombinant protein

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

NCBI and Uniprot Product Information

• NCBI GI #: 13027454
• NCBI GeneID: 78975
• NCBI Accession #: NP_076481.1
• NCBI GenBank Nucleotide #: NM_023991.1
• UniProt Accession #: Q09137
• Molecular Weight: 3,700 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-2
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, alpha 2 catalytic subunit
• NCBI Official Symbol: Prkaa2
• NCBI Official Synonym Symbols: Ampk; Ampka2
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-2
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-2
• UniProt Gene Name: Prkaa2
• UniProt Synonym Gene Names: Ampk; Ampk2; AMPK subunit alpha-2; ACACA kinase; HMGCR kinase
• UniProt Entry Name: AAPK2_RAT

NCBI Description

protein kinase that phosphorylates and inhibits acetyl-CoA carboxylase (ACC); rate-limiting enzyme in malonyl-CoA synthesis [RGD, Feb 2006]

Uniprot Description

AMPKA2: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of metabolic enzymes and transcription regulators. Regulates fatty acid synthesis by phosphorylating acetyl-CoA carboxylase. Regulates cholesterol synthesis by phosphorylating and inactivating hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Salicylate/aspirin directly activates kinase activity. Studies in the mouse suggest that AMPK2 may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Protein type: EC 2.7.11.1; Autophagy; EC 2.7.11.31; EC 2.7.11.27; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; CAMKL family; AMPK subfamily

Cellular Component: nucleoplasm; protein complex; apical plasma membrane; cytoplasm; nucleus; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; protein binding, bridging; protein serine/threonine kinase activity; protein binding; metal ion binding; [acetyl-CoA carboxylase] kinase activity; protein serine/threonine/tyrosine kinase activity; histone serine kinase activity; chromatin binding; ATP binding; protein kinase activity; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity

Biological Process: negative regulation of TOR signaling pathway; regulation of lipid metabolic process; Wnt receptor signaling pathway; transcription, DNA-dependent; protein heterooligomerization; lipid biosynthetic process; rhythmic process; regulation of circadian rhythm; glucose homeostasis; protein amino acid phosphorylation; cholesterol biosynthetic process; response to caffeine; cellular response to glucose starvation; cellular response to nutrient levels; regulation of transcription, DNA-dependent; fatty acid homeostasis; positive regulation of glycolysis; autophagy; response to activity; fatty acid biosynthetic process; positive regulation of autophagy; negative regulation of apoptosis