Rabbit anti-Human, Mouse ATP2A1 Polyclonal Antibody | anti-ATP2A1 antibody

ATP2A1 Antibody

Cat. Num. AAA9404662

• Gene Names: ATP2A1; ATP2A; SERCA1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: ATP2A1; Polyclonal Antibody; ATP2A1 Antibody; ATP2A; SERCA1; anti-ATP2A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total ATP2A1 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 2.2 mg/ml (varies by lot)
• Sequence Length: 869

• Applicable Applications for anti-ATP2A1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:200-1:1000; Immunohistochemistry: 1:50-1:200
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to residues near the C terminal of human ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• Target Name: ATP2A1
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 6%SDS-PAGE Lysates (from left to right): NIH/3T3 and LoVo cell Amount of lysate: 40ug per lane Primary antibody: 1/500 dilution Secondary antibody dilution: 1/8000 Exposure time: 3 minutes)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/35.)

Related Product Information for anti-ATP2A1 antibody

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms.

Product Categories/Family for anti-ATP2A1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 554506571
• NCBI GeneID: 487
• NCBI Accession #: NP_001273004.1
• NCBI GenBank Nucleotide #: NM_001286075.1
• UniProt Accession #: O14983; O14984; A8K5J9; B3KY17
• Molecular Weight: 95,199 Da
• NCBI Official Full Name: sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform c
• NCBI Official Synonym Full Names: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• NCBI Official Symbol: ATP2A1
• NCBI Official Synonym Symbols: ATP2A; SERCA1
• NCBI Protein Information: sarcoplasmic/endoplasmic reticulum calcium ATPase 1
• UniProt Protein Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
• Protein Family: Sarcoplasmic/endoplasmic reticulum calcium ATPase
• UniProt Gene Name: ATP2A1
• UniProt Synonym Gene Names: SERCA1; SR Ca(2+)-ATPase 1
• UniProt Entry Name: AT2A1_HUMAN

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013]

Uniprot Description

ATP2A1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Associated with sarcolipin (SLN) and phospholamban (PLN). Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression. Skeletal muscle, fast twitch muscle (type II) fibers. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.3.8; Membrane protein, multi-pass; Transporter; Transporter, ion channel; Endoplasmic reticulum; Membrane protein, integral; Hydrolase

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: I band; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; sarcoplasmic reticulum; membrane; perinuclear region of cytoplasm; integral to membrane; ER-Golgi intermediate compartment

Molecular Function: protein binding; protein homodimerization activity; calcium-transporting ATPase activity; calcium ion binding; ATP binding

Biological Process: apoptotic mitochondrial changes; metabolic process; positive regulation of fast-twitch skeletal muscle contraction; maintenance of mitochondrion localization; reduction of endoplasmic reticulum calcium ion concentration; elevation of endoplasmic reticulum calcium ion concentration; calcium ion transport; regulation of striated muscle contraction; blood coagulation; transmembrane transport; elevation of mitochondrial calcium ion concentration; negative regulation of striated muscle contraction

Disease: Brody Myopathy