Rabbit anti-Human, Mouse CASK Polyclonal Antibody | anti-CASK antibody

CASK, CT (Peripheral Plasma Membrane Protein CASK, hCASK, Calcium/Calmodulin-dependent Serine Protein Kinase, Protein Lin-2 Homolog, LIN2) (PE)

Cat. Num. AAA6466788

• Gene Names: CASK; CMG; FGS4; LIN2; TNRC8; CAGH39; CAMGUK; MICPCH; MRXSNA
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CASK; Polyclonal Antibody; CT (Peripheral Plasma Membrane Protein CASK; hCASK; Calcium/Calmodulin-dependent Serine Protein Kinase; Protein Lin-2 Homolog; LIN2) (PE); anti-CASK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Cask. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CASK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CASK (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CASK (arrow) using Cask Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CASK gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CASK antibody

Antibodies; Protein Kinases

References

1. Stevenson, D., et al., Mamm. Genome 11(10):934-937 (2000). 2. Cohen, A.R., et al., J. Cell Biol. 142(1):129-138 (1998). 3. Daniels, D.L., et al., Nat. Struct. Biol. 5(4):317-325 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 186972120
• NCBI GeneID: 8573
• NCBI Accession #: NP_003679
• NCBI GenBank Nucleotide #: NM_003688.3
• UniProt Accession #: O14936; O43215; Q17RI4; Q59HA0; Q5VT16; Q5VT17; Q5VT18; Q5VT19; Q66T42; Q9BYH6; A6NES1; B7ZKY0
• Molecular Weight: 103,211 Da
• NCBI Official Full Name: peripheral plasma membrane protein CASK isoform 1
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent serine protein kinase (MAGUK family)
• NCBI Official Symbol: CASK
• NCBI Official Synonym Symbols: CMG; FGS4; LIN2; TNRC8; CAGH39; CAMGUK; MICPCH; MRXSNA
• NCBI Protein Information: peripheral plasma membrane protein CASK; hCASK; protein lin-2 homolog; trinucleotide repeat containing 8; calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase
• UniProt Protein Name: Peripheral plasma membrane protein CASK
• Protein Family: Caskin
• UniProt Gene Name: CASK
• UniProt Synonym Gene Names: LIN2; hCASK
• UniProt Entry Name: CSKP_HUMAN

NCBI Description

This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, mental retardation and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked mental retardation. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

Uniprot Description

CASK: a MAGUK family protein and a protein kinase of the CAMK group. Contains one protein kinase domain, one guanylate kinase domain, two L27 domains, one PDZ domain and one SH3 domain. Plays a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Lacks the canonical DFG motif required for Mg++ binding, but retains protein kinase activity even without binding Mg++. Six alternatively spliced human isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Adaptor/scaffold; EC 2.7.11.1; Protein kinase, CAMK; CAMK group; CASK family

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: presynaptic membrane; nuclear lamina; focal adhesion; nuclear matrix; basolateral plasma membrane; cytoplasm; plasma membrane; nucleolus; basement membrane; intercellular junction; cytosol; actin cytoskeleton

Molecular Function: calmodulin binding; guanylate kinase activity; protein serine/threonine kinase activity; protein binding; neurexin binding; ATP binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; negative regulation of cell-matrix adhesion; protein amino acid phosphorylation; nucleotide phosphorylation

Disease: Mental Retardation And Microcephaly With Pontine And Cerebellar Hypoplasia; Fg Syndrome 4