Western Blot (WB)
(Figure 1. Western blot analysis of AMPK alpha 1/PRKAA1 using anti-AMPK alpha 1/PRKAA1 antibody (MBS1753385).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human MCF-7 whole cell lysatesLane 4: monkey Cos-7 whole cell lysatesLane 5: human K572 whole cell lysatesLane 6: human HEK293 whole cell lysatesLane 7: rat brain tissue lysatesLane 8: rat heart tissue lysatesLane 9: mouse brain tissue lysatesLane 10: mouse heart tissue lysatesLane 11: mouse intestines tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (Catalog # MBS1753385) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for AMPK alpha 1/PRKAA1 at approximately 64-66KD. The expected band size for AMPK alpha 1/PRKAA1 is at 64KD. )
Rabbit AMPK alpha 1/PRKAA1 Polyclonal Antibody | anti-PRKAA1 antibody
Anti-AMPK alpha 1/PRKAA1 Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
AMPK alpha 1/PRKAA1; Polyclonal Antibody; Anti-AMPK alpha 1/PRKAA1 Antibody; PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; EC 2. 7. 11. 31; Tau-protein kinase PRKAA1; aldehyde dehydrogenase 6 family member A1; anti-PRKAA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for AMPK alpha 1/PRKAA1 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-PRKAA1 antibody
Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
Application Notes
WB: 0.1-0.25ug/ml|Human, Mouse, Rat, Monkey|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Immunogen
E Coli-derived human AMPK alpha 1/PRKAA1 recombinant protein (Position: D359-P539).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of AMPK alpha 1/PRKAA1 using anti-AMPK alpha 1/PRKAA1 antibody (MBS1753385).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human MCF-7 whole cell lysatesLane 4: monkey Cos-7 whole cell lysatesLane 5: human K572 whole cell lysatesLane 6: human HEK293 whole cell lysatesLane 7: rat brain tissue lysatesLane 8: rat heart tissue lysatesLane 9: mouse brain tissue lysatesLane 10: mouse heart tissue lysatesLane 11: mouse intestines tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (Catalog # MBS1753385) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for AMPK alpha 1/PRKAA1 at approximately 64-66KD. The expected band size for AMPK alpha 1/PRKAA1 is at 64KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of AMPK alpha 1/PRKAA1 using anti-AMPK alpha 1/PRKAA1 antibody (MBS1753385).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human MCF-7 whole cell lysatesLane 4: monkey Cos-7 whole cell lysatesLane 5: human K572 whole cell lysatesLane 6: human HEK293 whole cell lysatesLane 7: rat brain tissue lysatesLane 8: rat heart tissue lysatesLane 9: mouse brain tissue lysatesLane 10: mouse heart tissue lysatesLane 11: mouse intestines tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (Catalog # MBS1753385) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for AMPK alpha 1/PRKAA1 at approximately 64-66KD. The expected band size for AMPK alpha 1/PRKAA1 is at 64KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-AMPK alpha 1/PRKAA1 antibody (MBS1753385).Overlay histogram showing SiHa cells stained with MBS1753385 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMPK alpha 1/PRKAA1 Antibody (MBS1753385, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-AMPK alpha 1/PRKAA1 antibody (MBS1753385).Overlay histogram showing SiHa cells stained with MBS1753385 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMPK alpha 1/PRKAA1 Antibody (MBS1753385, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Related Product Information for anti-PRKAA1 antibody
5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
References
1. "Entrez Gene: PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit".
2. Hawley SA, Davison M, Woods A, et al. (1996). "Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase. ". J. Biol. Chem. 271 (44): 27879-87.
2. Hawley SA, Davison M, Woods A, et al. (1996). "Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase. ". J. Biol. Chem. 271 (44): 27879-87.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
64,009 Da
NCBI Official Full Name
PRKAA1 protein
NCBI Official Synonym Full Names
protein kinase, AMP-activated, alpha 1 catalytic subunit
NCBI Official Symbol
PRKAA1
NCBI Official Synonym Symbols
AMPK; AMPKa1
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; AMPK alpha 1; HMGCR kinase; AMPK subunit alpha-1; tau-protein kinase PRKAA1; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; hydroxymethylglutaryl-CoA reductase
UniProt Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1
UniProt Gene Name
PRKAA1
UniProt Synonym Gene Names
AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
UniProt Entry Name
AAPK1_HUMAN
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Product Notes
The PRKAA1 prkaa1 (Catalog #AAA1753385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-AMPK alpha 1/PRKAA1 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's AMPK alpha 1/PRKAA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.1-0.25ug/ml|Human, Mouse, Rat, Monkey| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the PRKAA1 prkaa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPK alpha 1/PRKAA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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