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Western Blot (WB) (HBQ1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the HBQ1 antibody detected the HBQ1 protein (arrow).)

Rabbit anti-Human HBQ1 Polyclonal Antibody | anti-HBQ1 antibody

HBQ1, NT (HBQ1, Hemoglobin subunit theta-1, Hemoglobin theta-1 chain, Theta-1-globin) (Biotin)

Gene Names
HBQ1; HBQ
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HBQ1; Polyclonal Antibody; NT (HBQ1; Hemoglobin subunit theta-1; Hemoglobin theta-1 chain; Theta-1-globin) (Biotin); Theta-1-globin; anti-HBQ1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-HBQ1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
HBQ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HBQ1.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HBQ1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the HBQ1 antibody detected the HBQ1 protein (arrow).)

Western Blot (WB) (HBQ1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the HBQ1 antibody detected the HBQ1 protein (arrow).)

Immunohistochemistry (IHC)

(HBQ1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HBQ1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (HBQ1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HBQ1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-HBQ1 antibody
References
De Gobbi, M., et al. Science 312(5777):1215-1217(2006). Patrinos, G.P., et al. Hum. Mutat. 26(5):399-412(2005). Daniels, R.J., et al. Hum. Mol. Genet. 10(4):339-352(2001). Giardina, B., et al. Crit. Rev. Biochem. Mol. Biol. 30(3):165-196(1995) Kim, J.H., et al. Nucleic Acids Res. 17(14):5687-5700(1989).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,508 Da
NCBI Official Full Name
hemoglobin subunit theta-1
NCBI Official Synonym Full Names
hemoglobin, theta 1
NCBI Official Symbol
HBQ1
NCBI Official Synonym Symbols
HBQ
NCBI Protein Information
hemoglobin subunit theta-1; hemoglobin theta-1 chain; theta-1-globin
UniProt Protein Name
Hemoglobin subunit theta-1
Protein Family
UniProt Gene Name
HBQ1
UniProt Entry Name
HBAT_HUMAN

NCBI Description

Theta-globin mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue. The theta-1 gene may be expressed very early in embryonic life, perhaps sometime before 5 weeks. Theta-1 is a member of the human alpha-globin gene cluster that involves five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-2 -pseudoalpha-1 - alpha-2 - alpha-1 - theta-1 - 3'. Research supports a transcriptionally active role for the gene and a functional role for the peptide in specific cells, possibly those of early erythroid tissue. [provided by RefSeq, Jul 2008]

Uniprot Description

HBQ1: Theta-globin mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue. The theta-1 gene may be expressed very early in embryonic life, perhaps sometime before 5 weeks. Theta-1 is a member of the human alpha-globin gene cluster that involves five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-2 -pseudoalpha-1 - alpha-2 - alpha-1 - theta-1 - 3'. Research supports a transcriptionally active role for the gene and a functional role for the peptide in specific cells, possibly those of early erythroid tissue. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: hemoglobin complex

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: oxygen transport

Research Articles on HBQ1

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Product Notes

The HBQ1 hbq1 (Catalog #AAA6305627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HBQ1, NT (HBQ1, Hemoglobin subunit theta-1, Hemoglobin theta-1 chain, Theta-1-globin) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HBQ1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HBQ1 hbq1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HBQ1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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