Rabbit anti-Human AXL Polyclonal Antibody | anti-AXL antibody

AXL, NT (AXL, UFO, Tyrosine-protein kinase receptor UFO, AXL oncogene)

Cat. Num. AAA647702

• Gene Names: AXL; UFO; JTK11
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: AXL; Polyclonal Antibody; NT (AXL; UFO; Tyrosine-protein kinase receptor UFO; AXL oncogene); Anti -AXL; anti-AXL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-AXL antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: AXL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14~44 amino acids from the N-terminal region of human AXL.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in SKBR3 cell lysate using MBS647702. AXL (arrow) was detected using MBS647702. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue using MBS647702, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS647702 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of WiDr cells using MBS647702 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-AXL antibody

AXL, a member of the AXL/UFO subfamily of Tyr protein kinases, may function as a signal transducer between specific cell types of mesodermal origin. This Type I membrane protein has transforming potential in patients with chronic myeloproliferative disorder or chronic myelocytic leukemia. The protein contains 2 putative fibronectin type III domains and 2 putative immunoglobulin-like C2-type domains.

Product Categories/Family for anti-AXL antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 119577427
• NCBI GeneID: 558
• UniProt Accession #: P30530; Q8N5L2; Q9UD27
• Molecular Weight: 98,336 Da
• NCBI Official Full Name: AXL receptor tyrosine kinase, isoform CRA_b
• NCBI Official Synonym Full Names: AXL receptor tyrosine kinase
• NCBI Official Symbol: AXL
• NCBI Official Synonym Symbols: UFO; JTK11
• NCBI Protein Information: tyrosine-protein kinase receptor UFO; AXL oncogene; AXL transforming sequence/gene
• UniProt Protein Name: Tyrosine-protein kinase receptor UFO
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: AXL
• UniProt Synonym Gene Names: UFO
• UniProt Entry Name: UFO_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor. Ref.2 Ref.11 Ref.12 Ref.14 Ref.15 Ref.17 Ref.18 Ref.19 Ref.22

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation: Activated by GAS6-binding and subsequent autophosphorylation. Ref.9 Ref.17

Subunit structure: Heterodimer and heterotetramer with ligand GAS6. Interacts with CBL, GRB2, LCK, NCK2, PIK3R1, PIK3R2, PIK3R3, PLCG1, SOCS1 and TENC1. Part of a complex including AXL, TNK2 and GRB2, in which GRB2 promotes AXL recruitment by TNK2. Ref.8 Ref.9 Ref.10 Ref.13 Ref.16 Ref.20 Ref.23 Ref.24

Subcellular location: Cell membrane; Single-pass type I membrane protein Ref.7.

Tissue specificity: Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue. Ref.7

Post-translational modification: Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. Ref.10 Ref.20

Involvement in disease: AXL and its ligand GAS6 are highly expressed in thyroid carcinoma tissues, and might thus be involved in thyroid tumorigenesis. Overexpression of AXL and its ligand was also detected in many other cancers such as myeloproliferative disorders, prostatic carcinoma cells, or breast cancer.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.Contains 2 fibronectin type-III domains.Contains 2 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 protein kinase domain.