Western Blot (WB)
(Western blot analysis of AKAP12 using Jurkat whole cell lysates)
Rabbit anti-Human AKAP12 Polyclonal Antibody | anti-AKAP12 antibody
AKAP12 Antibody
Gene Names
AKAP12; SSeCKS; AKAP250
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
AKAP12; Polyclonal Antibody; AKAP12 Antibody; A kinase (PRKA) anchor protein (gravin) 12; A kinase Anchor Protein 12; A kinase anchor protein 250kDa; A-kinase anchor protein 12; A-kinase anchor protein 250 kDa; AKA12_HUMAN; AKAP 12; AKAP 250; AKAP-12; Akap12; AKAP250; DKFZp686M0430; DKFZp686O0331; FLJ20945; FLJ97621; Gercelin; Gravin; Kinase scaffold protein gravin; Myasthenia gravis autoantigen; Myasthenia gravis autoantigen gravin; OTTHUMP00000017426; OTTHUMP00000017427; Src-Suppressed C Kinase Substrate; Srcs5; SSeCKS; Tsga12; anti-AKAP12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
AKAP12 antibody detects endogenous levels of total AKAP12
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1782
Applicable Applications for anti-AKAP12 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human AKAP12
Subcellular Location
Cytoplasm > Cell Cortex. Cytoplasm > Cytoskeleton. May be part of the cortical cytoskeleton.
Tissue Specificity
Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
Predicted Cross Reactivity
Rabbit, Dog
Similarity
Rabbit (83%), Dog (86%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of AKAP12 using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis of AKAP12 using Jurkat whole cell lysates)
Related Product Information for anti-AKAP12 antibody
Function: Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Subunit Structure: Binds to dimeric RII-alpha regulatory subunit of PKC.
Similarity: Polybasic regions located between residues 266 and 557 are involved in binding PKC.
Subunit Structure: Binds to dimeric RII-alpha regulatory subunit of PKC.
Similarity: Polybasic regions located between residues 266 and 557 are involved in binding PKC.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 191 kDa
Predicted: 192 kDa
Predicted: 192 kDa
NCBI Official Full Name
A-kinase anchor protein 12 isoform 1
NCBI Official Synonym Full Names
A-kinase anchoring protein 12
NCBI Official Symbol
AKAP12
NCBI Official Synonym Symbols
SSeCKS; AKAP250
NCBI Protein Information
A-kinase anchor protein 12
UniProt Protein Name
A-kinase anchor protein 12
Protein Family
UniProt Gene Name
AKAP12
UniProt Synonym Gene Names
AKAP250; AKAP-12; AKAP 250
NCBI Description
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Research Articles on AKAP12
Similar Products
Product Notes
The AKAP12 akap12 (Catalog #AAA9609130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKAP12 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AKAP12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the AKAP12 akap12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKAP12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
