Testing Data
(Gel: 8%SDS-PAGE Lysate: 40ug HT-29 cell Primary antibody: 1/600 dilution Secondary antibody dilution: 1/8000 Exposure time: 40 seconds)
Rabbit anti-Human AKAP12 Polyclonal Antibody | anti-AKAP12 antibody
AKAP12 Antibody
Immunohistochemistry: 1:50-1:200
Testing Data
(Gel: 8%SDS-PAGE Lysate: 40ug HT-29 cell Primary antibody: 1/600 dilution Secondary antibody dilution: 1/8000 Exposure time: 40 seconds)
Testing Data
(Gel: 8%SDS-PAGE Lysate: 40ug HT-29 cell Primary antibody: 1/600 dilution Secondary antibody dilution: 1/8000 Exposure time: 40 seconds)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/40.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/40.)
NCBI and Uniprot Product Information
NCBI Description
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
AKAP12: a scaffold protein that contains 3 conserved PKA binding motifs. Suppresses podosome formation, inhibits RhoA and Cdc42 activity, and inhibits v-Src-induced oncogenic growth in NIH3T3. May inhibit Src-induced oncogenesis by reestablishing actin-based non-invasive cytoskeletal architecture.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 6q24-q25
Cellular Component: focal adhesion; cytoskeleton; cytoplasm; plasma membrane; cell cortex
Molecular Function: protein binding; protein kinase A binding; adenylate cyclase binding
Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of cAMP biosynthetic process; protein targeting
Research Articles on AKAP12
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Product Notes
The AKAP12 akap12 (Catalog #AAA9403093) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKAP12 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AKAP12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:200-1:1000 Immunohistochemistry: 1:50-1:200. Researchers should empirically determine the suitability of the AKAP12 akap12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKAP12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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