Rabbit anti-Human, Rat AKAP12 Polyclonal Antibody | anti-AKAP12 antibody

AKAP12 Rabbit Polyclonal

Cat. Num. AAA766484

• Gene Names: AKAP12; SSeCKS; AKAP250
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: AKAP12; Polyclonal Antibody; AKAP12 Rabbit Polyclonal; anti-AKAP12 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 1782

• Applicable Applications for anti-AKAP12 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: A kinase (PRKA) anchor protein 12
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon tissue slide using MBS766484 (AKAP12 Antibody) at dilution of 1:50)

Western Blot (WB)

(rat brain tissue were subjected to SDS PAGE followed by western blot with MBS766484 (AKAP12 Antibody) at dilution of 1:600)

NCBI and Uniprot Product Information

• NCBI GI #: 21493022
• NCBI GeneID: 9590
• NCBI Accession #: NP_005091.2
• NCBI GenBank Nucleotide #: NM_005100.3
• Molecular Weight: 180,990 Da
• NCBI Official Full Name: A-kinase anchor protein 12 isoform 1
• NCBI Official Synonym Full Names: A-kinase anchoring protein 12
• NCBI Official Symbol: AKAP12
• NCBI Official Synonym Symbols: SSeCKS; AKAP250
• NCBI Protein Information: A-kinase anchor protein 12
• UniProt Protein Name: A-kinase anchor protein 12
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP12
• UniProt Synonym Gene Names: AKAP250; AKAP-12; AKAP 250
• UniProt Entry Name: AKA12_HUMAN

NCBI Description

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

AKAP12: a scaffold protein that contains 3 conserved PKA binding motifs. Suppresses podosome formation, inhibits RhoA and Cdc42 activity, and inhibits v-Src-induced oncogenic growth in NIH3T3. May inhibit Src-induced oncogenesis by reestablishing actin-based non-invasive cytoskeletal architecture.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 6q24-q25

Cellular Component: cell cortex; cytoplasm; cytoskeleton; focal adhesion; plasma membrane

Molecular Function: adenylate cyclase binding; protein binding; protein kinase A binding

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of cAMP biosynthetic process; protein targeting