Rabbit anti-Human, Mouse PADI1 Polyclonal Antibody | anti-PADI1 antibody

PADI1 Polyclonal Antibody

Cat. Num. AAA9130516

• Gene Names: PADI1; PDI; PAD1; PDI1; HPAD10
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: PADI1; Polyclonal Antibody; PADI1 Polyclonal Antibody ; HPAD10;PAD1;PDI;PDI1; anti-PADI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 663

• Applicable Applications for anti-PADI1 antibody: Western Blot (WB)
• Application Notes: WB: 1:200 - 1:1000
• Immunogen: Recombinant protein of human PADI1
• Category: Primary antibody
• Observed MW: 65kDa
• Calculated MW: 74kDa
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PADI1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

Related Product Information for anti-PADI1 antibody

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogousgenes

Product Categories/Family for anti-PADI1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 122056685
• NCBI GeneID: 29943
• NCBI Accession #: NP_037490.2
• NCBI GenBank Nucleotide #: NM_013358.2
• UniProt Accession #: Q9ULC6; Q70SX6; A1L4K6
• NCBI Official Full Name: protein-arginine deiminase type-1
• NCBI Official Synonym Full Names: peptidyl arginine deiminase 1
• NCBI Official Symbol: PADI1
• NCBI Official Synonym Symbols: PDI; PAD1; PDI1; HPAD10
• NCBI Protein Information: protein-arginine deiminase type-1
• UniProt Protein Name: Protein-arginine deiminase type-1
• Protein Family: Protein-arginine deiminase
• UniProt Gene Name: PADI1
• UniProt Synonym Gene Names: PAD1; PDI1
• UniProt Entry Name: PADI1_HUMAN

NCBI Description

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008]

Uniprot Description

PADI1: Catalyzes the deimination of arginine residues of proteins. Epidermis, prostate, testis, placenta, spleen and thymus. Belongs to the protein arginine deiminase family.

Protein type: Hydrolase; EC 3.5.3.15

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: cytoplasm

Molecular Function: calcium ion binding; protein-arginine deiminase activity

Biological Process: peptidyl-citrulline biosynthetic process from peptidyl-arginine