Western Blot (WB)
(Western blot analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 antibody and ADD1 antibody (Ser726) (Lanes 3 and 4))
Rabbit ADD1 Polyclonal Antibody | anti-ADD1 antibody
ADD1 Antibody (Ser726)
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
ADD1; Polyclonal Antibody; ADD1 Antibody (Ser726); Rabbit Polyclonal ADD1 Antibody (Ser726); Polyclonal ADD1 antibody; Anti-ADD1 antibody; ADD-1 antibody; ADDA antibody; adducin 1 (alpha) antibody; Alpha-adducin antibody; erythrocyte adducin alpha subunit antibody; Erythrocyte adducin subunit alpha antibody; MGC3339 antibody; MGC44427 antibody; anti-ADD1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
ADD1 antibody (Ser726) detects endogenous levels of ADD1 only when phosphorylated at serine 726
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
768
Applicable Applications for anti-ADD1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
ADD1 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-S P-F-L) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 antibody and ADD1 antibody (Ser726) (Lanes 3 and 4))
Western Blot (WB)
(Western blot analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 antibody and ADD1 antibody (Ser726) (Lanes 3 and 4))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 antibody (Ser726))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 antibody (Ser726))
Related Product Information for anti-ADD1 antibody
ADD1 is a cytoskeletal protein that promotes the assembly of the spectrin-actin network. Adducin is a heterodimeric protein that consists of related subunits. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region.
Product Categories/Family for anti-ADD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
alpha-adducin isoform f
Protein Family
Similar Products
Product Notes
The ADD1 (Catalog #AAA5313616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ADD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the ADD1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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