Rabbit Adducin alpha/beta Polyclonal Antibody | anti-S726 antibody

Adducin alpha/beta (Phospho-S726/713) Polyclonal Antibody

Cat. Num. AAA3004495

• Gene Names: ADD1; ADDA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Adducin alpha/beta; Polyclonal Antibody; Adducin alpha/beta (Phospho-S726/713) Polyclonal Antibody; Alpha-adducin; Erythrocyte adducin subunit alpha; ADD1; ADDA; alpha-Adducin; Adducin1; Adducin-1; alpha Adducin; Adducin-alpha; Beta-adducin; Erythrocyte adducin subunit beta; ADD2; ADDB; beta-Adducin; beta Adducin; Adducin beta; Adducin-beta; anti-S726 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Adducin alpha/beta (S726/713) polyclonal antibody detects endogenous levels of Adducin alpha protein only when phosphorylated at Ser726 and Adducin beta protein only when phosphorylated at Ser713.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 737

• Applicable Applications for anti-S726 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human Adducin alpha around the phosphorylation site of Serine 726.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-Adducin alpha/beta (S726/713) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysate treated with PMALane2:SP2/0 cell lysate treated with PMALane3:PC12 cell lysate treated with PMA)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Adducin alpha/beta (phospho-S726/713) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Adducin alpha/beta (phospho-S726/713) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Western Blot (WB)

(Western Blot (WB) analysis of Adducin alpha/beta (phospho-S726/713) pAb at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:Hela treated with PMA (60ng/ml, 30min) whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:PC12 treated with PMA (60ng/ml, 30min) whole cell lysate (40ug))

Related Product Information for anti-S726 antibody

Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

NCBI and Uniprot Product Information

• NCBI GI #: 29826319
• NCBI GeneID: 118
• NCBI Accession #: NP_001110.2
• NCBI GenBank Nucleotide #: NP_001110.2
• UniProt Accession #: P35611; P35612
• Molecular Weight: ~ 81kDa
• NCBI Official Full Name: alpha-adducin isoform a
• NCBI Official Synonym Full Names: adducin 1
• NCBI Official Symbol: ADD1
• NCBI Official Synonym Symbols: ADDA
• NCBI Protein Information: alpha-adducin
• UniProt Protein Name: Alpha-adducin
• UniProt Gene Name: ADD1
• UniProt Synonym Gene Names: ADDA
• UniProt Entry Name: ADDA_HUMAN

NCBI Description

Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017]

Uniprot Description

ADD1: a cytoskeletal protein that promotes the assembly of the spectrin-actin network. Adducin is a heterodimeric protein that consists of related subunits. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Three alternatively-spliced isoforms have been described.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; F-actin capping protein complex; intermediate filament cytoskeleton; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; dendritic spine; cytosol; nucleus

Molecular Function: actin filament binding; calmodulin binding; protein homodimerization activity; spectrin binding; protein heterodimerization activity; T cell receptor binding; actin binding; structural molecule activity; transcription factor binding

Biological Process: actin filament bundle formation; positive regulation of protein binding; hemoglobin metabolic process; in utero embryonic development; unfolded protein response; apoptosis; multicellular organism growth; cell morphogenesis; positive regulation of endocytosis; positive regulation of angiogenesis; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; homeostasis of number of cells within a tissue; erythrocyte differentiation; barbed-end actin filament capping; cell volume homeostasis; actin cytoskeleton organization and biogenesis; cell structure disassembly during apoptosis

Disease: Hypertension, Essential