Rabbit ADD1 Polyclonal Antibody | anti-ADD1 antibody

ADD1 (Phospho-Ser726) Antibody

Cat. Num. AAA9408575

• Gene Names: ADD1; ADDA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: ADD1; Polyclonal Antibody; ADD1 (Phospho-Ser726) Antibody; ADDA; Erythrocyte adducin alpha subunit; anti-ADD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of ADD1only when phosphorylated at serine 726.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 737

• Applicable Applications for anti-ADD1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from Human ADD1.
• Modification: Phospho-Ser726
• Target Name: ADD1
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells using ADD1(Phospho-Ser726) Antibody and the same antibody preincubated with blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1(Phospho-Ser726) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using ADD1(Phospho-Ser726) Antibody.)

Related Product Information for anti-ADD1 antibody

Adducins are a family of cytoskeleton proteins encoded by three genes (a, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. a- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. a- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

Product Categories/Family for anti-ADD1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 29826319
• NCBI GeneID: 118
• NCBI Accession #: NP_001110.2
• NCBI GenBank Nucleotide #: NM_001119.4
• UniProt Accession #: P35611; Q13734; Q14729; Q16156; Q86XM2; Q9UJB6; A2A3N8; A2A3P0; B4DI79; D3DVR3; D3DVR4; D3DVR5
• Molecular Weight: 73,333 Da
• NCBI Official Full Name: alpha-adducin isoform a
• NCBI Official Synonym Full Names: adducin 1 (alpha)
• NCBI Official Symbol: ADD1
• NCBI Official Synonym Symbols: ADDA
• NCBI Protein Information: alpha-adducin
• UniProt Protein Name: Alpha-adducin
• Protein Family: Alpha-adducin
• UniProt Gene Name: ADD1
• UniProt Synonym Gene Names: ADDA
• UniProt Entry Name: ADDA_HUMAN

NCBI Description

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Uniprot Description

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.