Rabbit anti-Human, Mouse ADAM23 Polyclonal Antibody | anti-ADAM23 antibody

ADAM23 Polyclonal Antibody

Cat. Num. AAA3006372

• Gene Names: ADAM23; MDC3; MDC-3
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ADAM23; Polyclonal Antibody; ADAM23 Polyclonal Antibody; Disintegrin and metalloproteinase domain-containing protein 23; ADAM 23; Metalloproteinase-like; disintegrin-like; and cysteine-rich protein 3; MDC-3; ADAM-23; MDC3; anti-ADAM23 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ADAM23 polyclonal antibody detects endogenous levels of ADAM23 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 832

• Applicable Applications for anti-ADAM23 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to residues in Human ADAM23.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ADAM23 polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysateLane2:Raw264. 7 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ADAM23 pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing strong cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ADAM23 antibody

ADAMs (a disintegrin and metalloproteinase domain), also known as MDCs (metalloproteinase, disintegrin, and cysteine-rich domain) or cellular disintegrins, are a family of proteins that are expressed in numerous different tissues. They catalyze proteolysis, adhesion, fusion and intracellular signaling. ADAMs are membrane-anchored proteins, and there are over 30 different members in the family with many diverse functions. ADAM 23 is exclusively expressed in fetal and adult brain and may function as an integrin ligand in cells of neural origin. Adhesion of neuroblastoma and astrocytoma cells is promoted by the disentegrin-like domain of ADAM23 and is mediated by an interaction of this protein with alphavbeta3. A short amino acid sequence in the disintegrin loop of ADAM23 interacts with alphavbeta3 to mediate cell interactions that take place during both normal and pathological processes. The three characteristic histidine residues and the glutamic acid residue typical of metalloproteinases are lacking in the metalloproteinase-like domain of ADAM23. This suggests that ADAM23 may not be involved in protease-mediated events, but could exclusively serve in cell adhesion processes. In addition, evidence suggests that ADAM23 may be a tumor suppressor gene.

NCBI and Uniprot Product Information

• NCBI GI #: 4501913
• NCBI GeneID: 8745
• NCBI Accession #: NP_003803
• NCBI GenBank Nucleotide #: NP_003803.1
• UniProt Accession #: O75077
• Molecular Weight: ~ 92kDa
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 23 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 23
• NCBI Official Symbol: ADAM23
• NCBI Official Synonym Symbols: MDC3; MDC-3
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 23
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 23
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM23
• UniProt Synonym Gene Names: MDC3; ADAM 23; MDC-3
• UniProt Entry Name: ADA23_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. It is reported that inactivation of this gene is associated with tumorigenesis in human cancers. [provided by RefSeq, May 2013]