Typical Testing Data/Standard Curve (for reference only)
Human Interleukin 13 Receptor Alpha 2 (IL13Ra2) ELISA Kit | IL13Ra2 elisa kit
Human Interleukin 13 Receptor Alpha 2 (IL13Ra2) ELISA Kit
Gene Names
IL13RA2; CT19; IL-13R; IL13BP; CD213A2
Reactivity
Human
Synonyms
Interleukin 13 Receptor Alpha 2 (IL13Ra2); Human Interleukin 13 Receptor Alpha 2 (IL13Ra2) ELISA Kit; IL13Ra2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of IL13RA2. No significant cross-reactivity or interference between IL13RA2 and analogues was observed.
Sequence Length
380
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.129ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL13RA2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL13RA2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL13Ra2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL13RA2 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL13RA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL13RA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL13RA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL13RA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL13RA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL13RA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL13RA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL13RA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,176 Da
NCBI Official Full Name
interleukin-13 receptor subunit alpha-2
NCBI Official Synonym Full Names
interleukin 13 receptor subunit alpha 2
NCBI Official Symbol
IL13RA2
NCBI Official Synonym Symbols
CT19; IL-13R; IL13BP; CD213A2
NCBI Protein Information
interleukin-13 receptor subunit alpha-2
UniProt Protein Name
Interleukin-13 receptor subunit alpha-2
Protein Family
UniProt Gene Name
IL13RA2
UniProt Synonym Gene Names
IL13R; IL-13 receptor subunit alpha-2; IL-13R subunit alpha-2; IL-13R-alpha-2; IL-13RA2
NCBI Description
The protein encoded by this gene is closely related to Il13RA1, a subuint of the interleukin 13 receptor complex. This protein binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13. [provided by RefSeq, Jul 2008]
Uniprot Description
Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4).
Research Articles on IL13Ra2
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Product Notes
The Human IL13Ra2 il13ra2 (Catalog #AAA456093) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456093 ELISA Kit recognizes Human IL13Ra2. It is sometimes possible for the material contained within the vial of "Interleukin 13 Receptor Alpha 2 (IL13Ra2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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