Western Blot (WB)
(ACTA1/α-actin Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ACTA1/α-actin antibody detected the ACTA1/α-actin protein (arrow).)
Rabbit anti-Human Actin, alpha1 Polyclonal Antibody | anti-ACTN1 antibody
Actin, alpha1, CT (ACTA1, ACTA, Actin, alpha skeletal muscle)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution:
Western Blot: 1:000
Optimal dilutions to be determined by the reseacher.
Western Blot (WB)
(ACTA1/α-actin Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ACTA1/α-actin antibody detected the ACTA1/α-actin protein (arrow).)
Western Blot (WB)
(ACTA1/α-actin Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ACTA1/α-actin antibody detected the ACTA1/α-actin protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Subunit structure: Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP. Interacts with PSD. Interacts with MICALL2
By similarity. Ref.15 Ref.16 Ref.18 Ref.19
Subcellular location: Cytoplasm › cytoskeleton. Cytoplasm › myofibril › sarcomere › Z line. Cell membrane
By similarity. Cell junction
By similarity. Cell projection › ruffle
By similarity. Note: Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures
By similarity. Ref.14
Involvement in disease: Bleeding disorder, platelet-type 15 (BDPLT15) [MIM:615193]: An autosomal dominant form of macrothrombocytopenia. Affected individuals usually have no or only mild bleeding tendency, such as epistaxis. Laboratory studies show decreased numbers of large platelets and anisocytosis, but the platelets show no in vitro functional abnormalities.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.24
Sequence similarities: Belongs to the alpha-actinin family.Contains 1 actin-binding domain.Contains 2 CH (calponin-homology) domains.Contains 2 EF-hand domains.Contains 4 spectrin repeats.
Research Articles on ACTN1
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Product Notes
The ACTN1 actn1 (Catalog #AAA647659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Actin, alpha1, CT (ACTA1, ACTA, Actin, alpha skeletal muscle) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Actin, alpha1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot Dilution: Western Blot: 1:000 Optimal dilutions to be determined by the reseacher. Researchers should empirically determine the suitability of the ACTN1 actn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, alpha1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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